Immunofluorescence (IF)
(Immunofluorescence staining of mouse Dectin-1 in Raw264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse Dectin-1 monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).)
Rabbit anti-Mouse Dectin-1 Monoclonal Antibody | anti-CLEC7A antibody
Recombinant Anti-Dectin-1 Antibody, Rabbit Monoclonal
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence
Purity
Protein A
Synonyms
Dectin-1; Monoclonal Antibody; Recombinant Anti-Dectin-1 Antibody; Rabbit Monoclonal; Dectin-1/CLEC7A Antibody; Rabbit MAb; C-type lectin domain family 7; member A; Anti-beta-GR Antibody; Anti-BGR Antibody; Anti-Clecsf12 Antibody; anti-CLEC7A antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
010
Specificity
Mouse Dectin-1/CLEC7A
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CLEC7A antibody
Flow Cytometry (FC/FACS/FCM), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
ICC/IF: 1:20-1:100
FC/FACS/FCM: 1:25-1:100
FC/FACS/FCM: 1:25-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Mouse Dectin-1/CLEC7A protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Dectin-1/CLEC7A (rM Dectin-1/CLEC7A).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of mouse Dectin-1 in Raw264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse Dectin-1 monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).)
Immunofluorescence (IF)
(Immunofluorescence staining of mouse Dectin-1 in Raw264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse Dectin-1 monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Mouse Dectin-1(CLEC7A) expression on Raw264.7 cells. Cells were stained with purified anti-Mouse Dectin-1(CLEC7A), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Mouse Dectin-1(CLEC7A) expression on Raw264.7 cells. Cells were stained with purified anti-Mouse Dectin-1(CLEC7A), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Related Product Information for anti-CLEC7A antibody
Dectin-1 was recently identified as the most important receptor for beta-glucan. It is a type II transmembrane protein which binds beta-1, 3 and beta-1, 6 glucans, and is expressed on most cells of the innate immune system and has been implicated in phagocytosis as well as killing of fungi by macrophages, neutrophils and dendritic cells. Recognition of beta-glucan by dectin-1 triggers effective immune response, including phagocytosis and proinflammatory factor production, to eliminate infecting fungi, which especially benefits immunocompromised patients against opportunistic fungal infection. In addition, dectin-1 is involved in the adaptive immune response as well as autoimmune diseases and immune tolerance. Dectin-1 can recognize and respond to live fungal pathogens and is being increasingly appreciated as having a key role in the innate responses to these pathogens. In addition to its exogenous ligands, Dectin-1 can recognize an unidentified endogenous ligand on T cells and may act as a co-stimulatory molecule. Recent studies have highlighted the importance of Dectin-1 in anti-fungal immunity, in both mice and humans, and have suggested a possible involvement of this receptor in the control of mycobacterial infections.
Product Categories/Family for anti-CLEC7A antibody
References
Herre J, et al. (2004) The role of Dectin-1 in antifungal immunity. Crit Rev Immunol. 24(3): 193-203.Brown GD. (2006) Dectin-1: a signalling non-TLR pattern-recognition receptor. 6(1): 33-43.Sun L, et al. (2007) The biological role of dectin-1 in immune response. Int Rev Immunol. 26(5-6): 349-64.Schorey JS, et al. (2008) The pattern recognition receptor Dectin-1: from fungi to mycobacteria. Curr Drug Targets. 9(2): 123-9.Reid DM, et al. (2009) Pattern recognition: recent insights from Dectin-1. Curr Opin Immunol. 21(1): 30-7.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,420 Da
NCBI Official Full Name
C-type lectin domain family 7 member A
NCBI Official Synonym Full Names
C-type lectin domain family 7, member a
NCBI Official Symbol
Clec7a
NCBI Official Synonym Symbols
BGR; beta-GR; Clecsf12
NCBI Protein Information
C-type lectin domain family 7 member A; C-type (calcium dependent, carbohydrate recognition domain) lectin, superfamily member 12; C-type lectin superfamily member 12; DC-associated C-type lectin 1; beta-glucan receptor; dectin-1; dendritic cell-associate
Protein Family
Research Articles on CLEC7A
Similar Products
Product Notes
The CLEC7A (Catalog #AAA8111243) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-Dectin-1 Antibody, Rabbit Monoclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Dectin-1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS/FCM), Immunocytochemistry (ICC), Immunofluorescence (IF). ICC/IF: 1:20-1:100 FC/FACS/FCM: 1:25-1:100. Researchers should empirically determine the suitability of the CLEC7A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Dectin-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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