Dectin-1 Recombinant Protein | CLEC7A recombinant protein

Dectin-1 Protein, Human, Recombinant (mFc Tag)

Cat. Num. AAA8120113

• Gene Names: CLEC7A; BGR; CANDF4; DECTIN1; CLECSF12
• Purity: >90% as determined by SDS-PAGE
• Synonyms: Dectin-1; Dectin-1 Protein; Human; Recombinant (mFc Tag); Human Dectin-1/CLEC7A Protein (Fc Tag); BGR Protein; CANDF4 Protein; CLECSF12 Protein; DECTIN1 Protein; C-type lectin domain family 7; member A; CLEC7A recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Thr66-Met201

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Asp
• Tag: N-mouse IgG1-Fc
• Protein Construction: A DNA sequence encoding the human CLEC7A (NP_072092.2)(Thr66-Met201) was expressed with the Fc region of mouse IgG1 at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for CLEC7A recombinant protein

Background: Dectin-1 was recently identified as the most important receptor for beta-glucan. It is a type II transmembrane protein which binds beta-1,3 and beta-1,6 glucans, and is expressed on most cells of the innate immune system and has been implicated in phagocytosis as well as killing of fungi by macrophages, neutrophils and dendritic cells. Recognition of beta-glucan by dectin-1 triggers effective immune response, including phagocytosis and proinflammatory factor production, to eliminate infecting fungi, which especially benefits immunocompromised patients against opportunistic fungal infection. In addition, dectin-1 is involved in the adaptive immune response as well as autoimmune diseases and immune tolerance. Dectin-1 can recognize and respond to live fungal pathogens and is being increasingly appreciated as having a key role in the innate responses to these pathogens. In addition to its exogenous ligands, Dectin-1 can recognize an unidentified endogenous ligand on T cells and may act as a co-stimulatory molecule. Recent studies have highlighted the importance of Dectin-1 in anti-fungal immunity, in both mice and humans, and have suggested a possible involvement of this receptor in the control of mycobacterial infections.

Product Categories/Family for CLEC7A recombinant protein

ITIM/ITAM Immunoreceptors and Related Molecules

References

Herre J, et al. (2004) The role of Dectin-1 in antifungal immunity. Crit Rev Immunol. 24(3): 193-203.Brown GD. (2006) Dectin-1: a signalling non-TLR pattern-recognition receptor. 6(1): 33-43.Sun L, et al. (2007) The biological role of dectin-1 in immune response. Int Rev Immunol. 26(5-6): 349-64.Schorey JS, et al. (2008) The pattern recognition receptor Dectin-1: from fungi to mycobacteria. Curr Drug Targets. 9(2): 123-9.Reid DM, et al. (2009) Pattern recognition: recent insights from Dectin-1. Curr Opin Immunol. 21(1): 30-7.

NCBI and Uniprot Product Information

• NCBI GI #: 13384604
• NCBI GeneID: 64581
• NCBI Accession #: NP_072092.2
• NCBI GenBank Nucleotide #: NM_022570.4
• UniProt Accession #: Q9BXN2; Q6IPS7; Q96D32; Q96DR9; Q96LD3; Q96PA4; Q96PA5; Q96PA6; B2R861; B7Z494; B7Z5A9; B7Z5B9
• Molecular Weight: 27,627 Da
• NCBI Official Full Name: C-type lectin domain family 7 member A isoform b
• NCBI Official Synonym Full Names: C-type lectin domain family 7, member A
• NCBI Official Symbol: CLEC7A
• NCBI Official Synonym Symbols: BGR; CANDF4; DECTIN1; CLECSF12
• NCBI Protein Information: C-type lectin domain family 7 member A; dectin-1; beta-glucan receptor; lectin-like receptor 1; DC-associated C-type lectin 1; C-type lectin superfamily member 12; dendritic cell-associated C-type lectin 1; dendritic cell-associated C-type lectin-1; C-typ
• UniProt Protein Name: C-type lectin domain family 7 member A
• Protein Family: C-type lectin domain family
• UniProt Gene Name: CLEC7A
• UniProt Synonym Gene Names: BGR; CLECSF12; DECTIN1; DC-associated C-type lectin 1; Dectin-1
• UniProt Entry Name: CLC7A_HUMAN

NCBI Description

This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. The encoded glycoprotein is a small type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif. It functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq, Jul 2008]

Uniprot Description

dectin-1: Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation. Up-regulated during differentiation from monocytes into dendritic cells. Isoform 5 interacts with RANBP9. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.2

Cellular Component: cytoplasm; plasma membrane; integral to membrane

Molecular Function: metal ion binding; carbohydrate binding; MHC protein binding; pattern recognition receptor activity

Biological Process: T cell activation; innate immune response; carbohydrate mediated signaling; phagocytosis, recognition; cell recognition; pattern recognition receptor signaling pathway; defense response to protozoan; inflammatory response

Disease: Candidiasis, Familial, 4; Aspergillosis, Susceptibility To