Heat Shock Cognate Protein 70, Bovine Recombinant Protein
Heat Shock Cognate Protein 70, Recombinant, Bovine (HSC70, Heat Shock Protein 73, HSP73)
Applications
Western Blot
Purity
Purified~85% pure as determined by SDS-PAGE analysis.
Synonyms
Heat Shock Cognate Protein 70; Bovine; Recombinant; Bovine (HSC70; Heat Shock Protein 73; HSP73); Bovine recombinant protein
Host
Recombinant, Bovine (E Coli)
Purity/Purification
Purified
~85% pure as determined by SDS-PAGE analysis.
~85% pure as determined by SDS-PAGE analysis.
Form/Format
Supplied as a liquid in 10mM Tris-HCl, pH 7.5, 300mM sodium chloride, 2mM DTT, 1mM EDTA.
Applicable Applications for Heat Shock Cognate Protein 70, Bovine recombinant protein
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Dilute in an SDS containing sample buffer (e.g. Laemmli, U.K., (1970) Nature 277: 680-685) and heated at 100 degree C for 5 minutes prior to use. On a 15-well minigel system, 100ng of protein per lane should be sufficient when used in a colorimetric Western Blot. This protein does not contain inducible hsp70 or DnaK as judged by Western Blot analysi
Dilution: Dilute in an SDS containing sample buffer (e.g. Laemmli, U.K., (1970) Nature 277: 680-685) and heated at 100 degree C for 5 minutes prior to use. On a 15-well minigel system, 100ng of protein per lane should be sufficient when used in a colorimetric Western Blot. This protein does not contain inducible hsp70 or DnaK as judged by Western Blot analysi
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.
Related Product Information for Heat Shock Cognate Protein 70, Bovine recombinant protein
The 70kD heat shock cognate protein, hsc70, is closely related biochemically and biologically to hsp70 and is part of the hsp70 family which contains a number of highly-related protein isoforms ranging in size from 66kD to 78kD. These proteins include both cognate members which are found within major intracellular compartments and highly inducible isoforms which appear to be predominantly cytoplasmic or nuclear in distribution (1). Members of the hsp70 family are molecular chaperones which are involved in many cellular functions such as protein folding, transport, maturation and degradation and they exert their function in an ATP-dependent manner. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding and the binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (2). Recent data has demonstrated that BAG-1 which possesses a ubiquitin-like domain at its amino terminus modulates the chaperone activity of hsc70 and hsp70. These findings reveal a role of BAG-1 as a physical link between the hsc70/hsp70 chaperone system and the proteasome (3). There is also experimental data which shows that the ATPase domain and the substrate binding domain of hsc70 cooperate and form a co-chaperone-chaperone complex with the synaptic vesicle cysteine string protein (csp) which is essential for normal neurotransmitter release (4).
Product Categories/Family for Heat Shock Cognate Protein 70, Bovine recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
heat shock cognate protein 70
Similar Products
Product Notes
The Heat Shock Cognate Protein 70, Bovine (Catalog #AAA637557) is a Recombinant Protein produced from Recombinant, Bovine (E Coli) and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Heat Shock Cognate Protein 70, Bovine can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Dilute in an SDS containing sample buffer (e.g. Laemmli, U.K., (1970) Nature 277: 680-685) and heated at 100 degree C for 5 minutes prior to use. On a 15-well minigel system, 100ng of protein per lane should be sufficient when used in a colorimetric Western Blot. This protein does not contain inducible hsp70 or DnaK as judged by Western Blot analysi. Researchers should empirically determine the suitability of the Heat Shock Cognate Protein 70, Bovine for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Heat Shock Cognate Protein 70, Bovine, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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