Typical Testing Data/Standard Curve (for reference only)
Human Migration And Invasion Enhancer 1 (MIEN1) ELISA Kit | MIEN1 elisa kit
Human Migration And Invasion Enhancer 1 (MIEN1) ELISA Kit
Gene Names
MIEN1; C35; ORB3; XTP4; RDX12; C17orf37
Reactivity
Human
Synonyms
Migration And Invasion Enhancer 1 (MIEN1); Human Migration And Invasion Enhancer 1 (MIEN1) ELISA Kit; RDX12; C35; ORB3; XTP4; C17orf37; Redox Protein 12kDa; HBV X-transactivated gene 4 protein; HBV XAg-transactivated protein 4; MIEN1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Migration And Invasion Enhancer 1 (MIEN1).
No significant cross-reactivity or interference between Migration And Invasion Enhancer 1 (MIEN1) and analogues was observed.
No significant cross-reactivity or interference between Migration And Invasion Enhancer 1 (MIEN1) and analogues was observed.
Sequence Length
115
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates, Cell lysates and Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.056ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Migration And Invasion Enhancer 1 (MIEN1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Migration And Invasion Enhancer 1 (MIEN1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MIEN1 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Migration And Invasion Enhancer 1 (MIEN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Migration And Invasion Enhancer 1 (MIEN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Migration And Invasion Enhancer 1 (MIEN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Migration And Invasion Enhancer 1 (MIEN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for MIEN1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
12,403 Da
NCBI Official Full Name
migration and invasion enhancer 1 isoform 1
NCBI Official Synonym Full Names
migration and invasion enhancer 1
NCBI Official Symbol
MIEN1
NCBI Official Synonym Symbols
C35; ORB3; XTP4; RDX12; C17orf37
NCBI Protein Information
migration and invasion enhancer 1
UniProt Protein Name
Migration and invasion enhancer 1
Protein Family
UniProt Gene Name
MIEN1
UniProt Synonym Gene Names
C17orf37; RDX12; XTP4
Uniprot Description
MIEN1: Increases cell migration by inducing filopodia formation at the leading edge of migrating cells. Plays a role in regulation of apoptosis, possibly through control of CASP3. May be involved in a redox-related process. Belongs to the SelWTH family.
Protein type: Apoptosis; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 17q12
Cellular Component: cytosol
Molecular Function: protein binding
Biological Process: apoptosis; negative regulation of apoptosis; positive regulation of cell migration; positive regulation of filopodium formation
Research Articles on MIEN1
1. Down-regulation of C35 decreased the cell viability and migration of breast ductal carcinoma cells.
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Product Notes
The Human MIEN1 mien1 (Catalog #AAA2098132) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2098132 ELISA Kit recognizes Human MIEN1. It is sometimes possible for the material contained within the vial of "Migration And Invasion Enhancer 1 (MIEN1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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