SDS-Page
(SDS-PAGE analysis of DnaK: Lane 1: MWM, Lanes 2-5: 0.5, 1, 2, 5ug of D4015-11)
DnaK, E. coli Recombinant Protein
DnaK, Recombinant, E. coli
Applications
Western Blot
Purity
Highly Purified95% as determined by SDS-PAGE analysis.
Synonyms
DnaK; E. coli; Recombinant; E. coli recombinant protein
Host
E Coli
Purity/Purification
Highly Purified
95% as determined by SDS-PAGE analysis.
95% as determined by SDS-PAGE analysis.
Form/Format
Supplied as a liquid in 40mM Tris, pH 7.5, 80mM sodium chloride, 0.8mM DTT, 0.08mM PMSF, 20% glycerol.
Applicable Applications for DnaK, E. coli recombinant protein
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.. Other applications not tested
Dilution: Western Blot: dilute into appropriate volume of an SDS containing sample buffer and heated at 100 degree C for 5 minutes prior to use. On a 15-well minigel system, 100ng of protein per lane should be sufficient when used with a colorimetric system.
Functional Activity Assay:
This protein has tested positive for ATP hydrolysis in an ATPase assay.
Dilution: Western Blot: dilute into appropriate volume of an SDS containing sample buffer and heated at 100 degree C for 5 minutes prior to use. On a 15-well minigel system, 100ng of protein per lane should be sufficient when used with a colorimetric system.
Functional Activity Assay:
This protein has tested positive for ATP hydrolysis in an ATPase assay.
Preparation and Storage
For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
SDS-Page
(SDS-PAGE analysis of DnaK: Lane 1: MWM, Lanes 2-5: 0.5, 1, 2, 5ug of D4015-11)
SDS-Page
(SDS-PAGE analysis of DnaK: Lane 1: MWM, Lanes 2-5: 0.5, 1, 2, 5ug of D4015-11)
Related Product Information for DnaK, E. coli recombinant protein
DnaK is an acidic 70kD Escherichia coli heat shock protein which belongs to the molecular chaperone class of proteins (1). The sequence of the dnaK gene is highly homologous with eukaryotic Hsp70 proteins. Reversible binding of Hsp70 to unfolded nascent polypeptides, partially denatured proteins and also some native proteins leads to: i) the stabilization of polypeptide chains during de novo folding, ii) protection of other proteins from aggregation, iii) reactivation of heat inactivated enzymes, iv) promotion of peptide translocation through membranes, and v) exposure of partially denatured proteins for efficient proteolysis. The N-terminal domains of Hsp70 proteins contain an ATPase active site, and the C-terminal domains contain a peptide binding site. DnaK has been shown to bind to various aberrant proteins including protein fragments, mutant proteins, unfolded proteins as well as native proteins: lambdaP, lambdaO and sigma32. DnaK's ATPase activity is stimulated by aromatic and aliphatic amino acids. The E. coli DnaK protein can be autophosphorylated, and like all Hsp70 members possesses a weak ATPase activity (1). The ADP created during this reaction remains bound to DnaK and can be released by GrpE co-chaperone protein. DnaK's ATPase activity is stimulated up to 50 times by the joint presence of the co-chaperones DnaJ and GrpE heat shock proteins. DnaK changes its conformation in the presence of ATP, triggering release of the protein substrate from the DnaK complex. Many in vitro functions of DnaK protein are potentiated by cooperation with the E. coli molecular chaperones DnaJ and GrpE:
Product Categories/Family for DnaK, E. coli recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
DnaK protein (heat shock protein 70)
Similar Products
Product Notes
The DnaK, E. coli (Catalog #AAA635688) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's DnaK, E. coli can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Other applications not tested Dilution: Western Blot: dilute into appropriate volume of an SDS containing sample buffer and heated at 100 degree C for 5 minutes prior to use. On a 15-well minigel system, 100ng of protein per lane should be sufficient when used with a colorimetric system. Functional Activity Assay: This protein has tested positive for ATP hydrolysis in an ATPase assay. Researchers should empirically determine the suitability of the DnaK, E. coli for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DnaK, E. coli, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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