Heat Shock Protein 71, Mycobacterium tuberculosis Recombinant Protein
Heat Shock Protein 71, Recombinant, Mycobacterium tuberculosis (HSP71)
Applications
Western Blot
Purity
Highly Purified90% as determined by SDS-PAGE analysis.
Synonyms
Heat Shock Protein 71; Mycobacterium tuberculosis; Recombinant; Mycobacterium tuberculosis (HSP71); Mycobacterium tuberculosis recombinant protein
Host
E Coli
Purity/Purification
Highly Purified
90% as determined by SDS-PAGE analysis.
90% as determined by SDS-PAGE analysis.
Form/Format
Supplied as a liquid in 0.85% sodium chloride, 0.084% Triton X-114.
Applicable Applications for Heat Shock Protein 71, Mycobacterium tuberculosis recombinant protein
Western Blot (WB)
Application Notes
Suitable for use in Western Blot analysis and is not suitable for other applications. For Westerns, resuspend the protein in 20ul (1ug/ul) of a 1X SDS-PAGE sample buffer (6). On a 15-well minigel system, 100ng of protein per lane should be sufficient when used in a colorimetric Western Blot.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.
Related Product Information for Heat Shock Protein 71, Mycobacterium tuberculosis recombinant protein
The 71kD antigen from M. tuberculosis is recognized by antibodies and by T lymphocytes during infection (1) and sequence analysis indicates a relationship between this antigen and the highly conserved Hsp70 family (2). Biochemical as well as serological characterization of the M. tuberculosis 71kD antigen confirms that it is a member of the Hsp70 family and metabolic labeling demonstrates that it is a major component of the mycobacterial response to heat stress. M. tuberculosis Hsp71 may have a biochemical role similar to that of DnaK which is involved in ATP-dependent protein-protein interactions and like eukaryotic Hsp70 homologs, is thought to participate in the assembly/disassembly of protein complexes (3, 4). Several studies have shown that during cellular rejection, the allograft undergoes a stress response which regulates the expression of stress proteins and triggers the recruitment and activation of hsp-reactive lymphocytes. Recent data using the rat model of heterotropic heart transplants, demonstrates that allograft-infiltrating lymphocytes respond to recombinant mycobacterial hsp71 and irradiated syngenic spleen cells as a source of self-APC. The hsp71-dependency of self-APC reactive T cell reactivity represents a previously unrecognized mechanism of cellular immunity to allografts. This mechanism might be related to the peptide binding properties of hsp71 and the ability of stress proteins to function as molecular chaperones in antigen processing (5).
Product Categories/Family for Heat Shock Protein 71, Mycobacterium tuberculosis recombinant protein
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Product Notes
The Heat Shock Protein 71, Mycobacterium tuberculosis (Catalog #AAA636690) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Heat Shock Protein 71, Mycobacterium tuberculosis can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot analysis and is not suitable for other applications. For Westerns, resuspend the protein in 20ul (1ug/ul) of a 1X SDS-PAGE sample buffer (6). On a 15-well minigel system, 100ng of protein per lane should be sufficient when used in a colorimetric Western Blot. Researchers should empirically determine the suitability of the Heat Shock Protein 71, Mycobacterium tuberculosis for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Heat Shock Protein 71, Mycobacterium tuberculosis, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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