Western Blot (WB)
((0.3ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat TBC1D9 Polyclonal Antibody | anti-TBC1D9 antibody
Goat anti-TBC1D9 Antibody
Gene Names
TBC1D9; MDR1; GRAMD9
Reactivity
Tested: Human, Pig; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
TBC1D9; Polyclonal Antibody; Goat anti-TBC1D9 Antibody; Goat Anti-TBC1D9 (aa444-458) Antibody; TBC1 domain family; member 9 (with GRAM domain); MDR1; TBC1 domain family member 9; TBC1 domain family member 9A; TBC1D9 antibody; member 9 (with GRAM domain) antibody; MDR1 antibody; TBC1 domain family member 9 antibody; TBC1 domain family member 9A antibody; anti-TBC1D9 antibody
Host
Goat
Reactivity
Tested: Human, Pig; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
EDKLHCEDIGEDT
Sequence Length
1266
Applicable Applications for anti-TBC1D9 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Experiments gave bands at approx 90kDa and 55kDa in lysates of cell line HeLa. and in Pig Brain lysates. The sum of the observed sizes equals 145kDa. This protein has a calculated MW of 143kDa according to Human NP_055945.2 and Pig XP_005666914.2). Recommended concentration: 0.3-1ug/ml.
Western Blot: Experiments gave bands at approx 90kDa and 55kDa in lysates of cell line HeLa. and in Pig Brain lysates. The sum of the observed sizes equals 145kDa. This protein has a calculated MW of 143kDa according to Human NP_055945.2 and Pig XP_005666914.2). Recommended concentration: 0.3-1ug/ml.
Immunogen
Peptide with sequence EDKLHCEDIGEDT, from the internal region (near C terminus) of the protein sequence according to NP_055945.2.
Epitope
Internal region (near C Terminus)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.3ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of Pig Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of Pig Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
143,229 Da
NCBI Official Full Name
TBC1 domain family member 9
NCBI Official Synonym Full Names
TBC1 domain family member 9
NCBI Official Symbol
TBC1D9
NCBI Official Synonym Symbols
MDR1; GRAMD9
NCBI Protein Information
TBC1 domain family member 9
UniProt Protein Name
TBC1 domain family member 9
Protein Family
UniProt Gene Name
TBC1D9
UniProt Synonym Gene Names
KIAA0882; TBC1D9A
UniProt Entry Name
TBCD9_HUMAN
Uniprot Description
TBC1D9: May act as a GTPase-activating protein for Rab family protein(s).
Protein type: GAPs; GAPs, Rab
Chromosomal Location of Human Ortholog: 4q31.21
Cellular Component: endomembrane system; intracellular
Molecular Function: calcium ion binding; GTPase activator activity; protein binding; Rab GTPase binding
Biological Process: intracellular protein transport; regulation of vesicle fusion
Research Articles on TBC1D9
Similar Products
Product Notes
The TBC1D9 tbc1d9 (Catalog #AAA423365) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-TBC1D9 Antibody reacts with Tested: Human, Pig; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's TBC1D9 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Experiments gave bands at approx 90kDa and 55kDa in lysates of cell line HeLa. and in Pig Brain lysates. The sum of the observed sizes equals 145kDa. This protein has a calculated MW of 143kDa according to Human NP_055945.2 and Pig XP_005666914.2). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the TBC1D9 tbc1d9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: EDKLHCEDIG EDT. It is sometimes possible for the material contained within the vial of "TBC1D9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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