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Hemopexin Human Active Protein | HPX active protein

Hemopexin, Recombinant Human (HPX, HX, Beta-1B-glycoprotein, FLJ56652, Hemo)

Gene Names
HPX; HX
Purity
Purified
90%, as determined by SDS-PAGE and visualized by silver stain.
Synonyms
Hemopexin Human; Hemopexin; Recombinant Human (HPX; HX; Beta-1B-glycoprotein; FLJ56652; Hemo); HPX active protein
Ordering
For Research Use Only!
Purity/Purification
Purified
90%, as determined by SDS-PAGE and visualized by silver stain.
Form/Format
Supplied as a liquid in 25mM MES, 0.15M NaCl, pH 6.5.
Activity
Measured by its ability to bind protoporphyrin IX (PPP-IX). See Assay Protocol on the next page for details. Under the described conditions, rhHemopexin binds > 6uM PPP-IX, resulting in 50% decrease in the fluorescence signal of rhHemopexin.
Endotoxin Level
1EU/ug of the enzyme as determined by the LAL method.
Preparation and Storage
Samples are stable for up to six months at -20 to -70 degree C from the date of receipt.
Upon receiving, the protein can be aliquoted and stored at -20 to -70 degree C in a manual defrost freezer for three months without a significant loss of activity. Avoid repeated freeze-thaw cycles.
Related Product Information for HPX active protein
Hemopexin (HPX) is a 60kD plasma glycoprotein with two four-bladed b-propeller folds. This structural motif has been found in other proteins including collagenases and provides sites for protein-protein interactions (1-3). The liver is the major synthesizing organ. Expression in the central nervous system, in the retina, and in peripheral nerves has also been observed. Hemopexin belongs to the family of the acute-phase proteins whose synthesis is induced after an inflammatory event. Hemopexin participates in maintaining and recycling the iron pool by utilizing its high binding affinity toward heme composed of protoporphyrin IX and iron. It also functions in preventing oxidation caused by heme after hemolysis. Hydrophobic heme molecules can intercalate into lipid membranes and participate in the oxidation of lipid membrane components through the Fenton reaction resulting in lipid peroxidation. Hemopexin undergoes a conformational change upon the binding of heme. The conformational change allows hemopexin to interact with a specific receptor, forming a complex which is then internalized. In the plasma, It is likely that heme binds albumin (35-55 g/L) first because of the higher concentration of albumin in plasma than hemopexin (0.5-1.2 g/L), and is then transferred to hemopexin, which has much higher affinity (Kd ~ 1 pM) toward heme. Heme concentrations in plasma increase after hemolysis, which is associated with several pathological conditions such as reperfusion injury and ischemia.

The purified, secreted rhHemopexin has the N-terminal sequence of T24PLPPTSAHG. The 445 amino acid residue rhHemopexin predicts a molecular mass of 50kD and migrates as a 71kD band in SDS-PAGE under reducing conditions.
Product Categories/Family for HPX active protein

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
51,676 Da
NCBI Official Full Name
hemopexin
NCBI Official Synonym Full Names
hemopexin
NCBI Official Symbol
HPX
NCBI Official Synonym Symbols
HX
NCBI Protein Information
hemopexin; beta-1B-glycoprotein
UniProt Protein Name
Hemopexin
Protein Family
UniProt Gene Name
HPX
UniProt Entry Name
HEMO_HUMAN

NCBI Description

This gene encodes a plasma glycoprotein that binds heme with high affinity. The encoded protein is an acute phase protein that transports heme from the plasma to the liver and may be involved in protecting cells from oxidative stress. [provided by RefSeq, Apr 2009]

Uniprot Description

HPX: Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation. Belongs to the hemopexin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11p15.5-p15.4

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; metal ion binding; heme transporter activity

Biological Process: receptor-mediated endocytosis; positive regulation of humoral immune response mediated by circulating immunoglobulin; heme transport; positive regulation of immunoglobulin production; positive regulation of tyrosine phosphorylation of Stat1 protein; viral reproduction; cellular iron ion homeostasis; hemoglobin metabolic process; heme metabolic process

Research Articles on HPX

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Product Notes

The HPX hpx (Catalog #AAA636541) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Hemopexin Human, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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