Cholesterol esterase Mouse Active Protein | Cel active protein

Cholesterol esterase, Recombinant Mouse (Carboxyl Ester Lipase, CEL, Lysophosphatidylcholine, Bile salt-stimulated lipase, BAL, BSDL, BSSL, Bucelipase, CEase, CELL,, lysoPC, PTL, Pancreatic triglyceride lipase, FAP, FAPP, LIPA, MODY8, Pancreatic lysophosp

Cat. Num. AAA635625

• Gene Names: Cel; BAL; 1810036E18Rik
• Reactivity: Mouse
• Applications: ELISA
• Purity: Highly Purified; 90% by SDS­PAGE under reducing conditions and visualized by silver stain.
• Synonyms: Cholesterol esterase Mouse; Cholesterol esterase; Recombinant Mouse (Carboxyl Ester Lipase; CEL; Lysophosphatidylcholine; Bile salt-stimulated lipase; BAL; BSDL; BSSL; Bucelipase; CEase; CELL; ; lysoPC; PTL; Pancreatic triglyceride lipase; FAP; FAPP; LIPA; MODY8; Pancreatic lysophosp; Pancreatic lysophos; Cel active protein

• Reactivity: Mouse
• Purity/Purification: Highly Purified; 90% by SDS­PAGE under reducing conditions and visualized by silver stain.
• Form/Format: Supplied as a 0.2um sterile-filtered solution in Tris and sodium chloride.

• Applicable Applications for Cel active protein: ELISA (EL/EIA)
• Activity: Measured by its ability to cleave p-Nitrophenylbutyrate (PNPB).; The specific activity is 4,000 pmol/min/ug, as measured under the described conditions. See Activity Assay Protocol.; Specific Activity (pmoles/min/ug) = Adjusted Abs* (OD) x Conversion Factor** (pmole/OD) Incubation time (min) x amount of enzyme (ug)
• Endotoxin Level: 1.0 EU per 1ug of the protein by the LAL method.
• Preparation and Storage: 12 months from date of receipt, 20 to 70 degree C as supplied.; 1 month, 2 to 8 degree C under sterile conditions after reconstitution.; 3 months, 20 to 70 degree C under sterile conditions after reconstitution.; Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Related Product Information for Cel active protein

Carboxyl ester lipase, also known as bile salt­stimulated lipase, belongs to the Type­B carboxyl esterase/lipase family of enzymes. The enzyme possesses a Ser­His­Asp catalytictriad, and is capable of hydrolyzing both ester and amide bonds. CEL is a non­specificlipase which hydrolyzes a variety of substrates, including cholesterylesters, acylglycerols, and ceramide (1). CEL is highly expressed by pancreatic acinar cells and secreted into the gastrointestinal tract, where it contributes to the digestion of dietary lipids (2). It is expressed at much lower levels by the liver, macrophages, and endothelial cells (3). CEL is present in the circulation, where it may play a role in low densi ty lipoprotein metabolism and atherogenesis (4).

Product Categories/Family for Cel active protein

Molecular Biology; MB-Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 1046363
• NCBI GeneID: 12613
• UniProt Accession #: Q64285
• Molecular Weight: 65,813 Da
• NCBI Official Full Name: cholesterol esterase
• NCBI Official Synonym Full Names: carboxyl ester lipase
• NCBI Official Symbol: Cel
• NCBI Official Synonym Symbols: BAL; 1810036E18Rik
• NCBI Protein Information: bile salt-activated lipase; BSSL; sterol esterase; cholesterol esterase; bile salt-stimulated lipase; pancreatic lysophospholipase
• UniProt Protein Name: Bile salt-activated lipase
• Protein Family: Bile salt-activated lipase
• UniProt Gene Name: Cel
• UniProt Synonym Gene Names: Lip1; BAL; BSSL
• UniProt Entry Name: CEL_MOUSE

Uniprot Description

CEL: Catalyzes fat and vitamin absorption. Acts in concert with pancreatic lipase and colipase for the complete digestion of dietary triglycerides. Defects in CEL are a cause of maturity-onset diabetes of the young type 8 with exocrine dysfunction (MODY8); also known as diabetes and pancreatic exocrine dysfunction (DPED). MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. Belongs to the type-B carboxylesterase/lipase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Lipid Metabolism - glycerolipid; Lipase; Lipid Metabolism - steroid biosynthesis; Secreted; EC 3.1.1.3; EC 3.1.1.13

Cellular Component: Golgi apparatus; extracellular space; protein complex; rough endoplasmic reticulum; intracellular membrane-bound organelle; cytoplasm; extracellular region; cytosol; lipid raft

Molecular Function: sterol esterase activity; retinyl-palmitate esterase activity; triacylglycerol lipase activity; hydrolase activity; protein complex binding; glycosphingolipid binding; lysophospholipase activity

Biological Process: retinol metabolic process; ceramide catabolic process; lipid metabolic process; pancreatic juice secretion; cholesterol catabolic process; lipid catabolic process