CEL recombinant protein

CEL Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122844

• Gene Names: Cel; BAL; 1810036E18Rik
• Purity: >90% as determined by SDS-PAGE
• Synonyms: CEL; CEL Protein; Mouse; Recombinant (His Tag); Mouse CEL/Bile salt-activated lipase Protein (His Tag); 1810036E18Rik Protein; BAL Protein; carboxyl ester lipase; CEL recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Leu534

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ala 21
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse CEL (NP_034015.1)(Met1-Leu534) was expressed with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CEL recombinant protein

Background: CEL-maturity onset diabetes of the young (MODY), diabetes with pancreatic lipomatosis and exocrine dysfunction, is due to dominant frameshift mutations in the acinar cell carboxyl ester lipase gene (CEL). Bile-salt activated carboxylic ester lipase (CEL) is a major triglyceride, cholesterol ester and vitamin ester hydrolytic enzyme contained within pancreatic and lactating mammary gland secretions. Carboxyl ester lipase is a digestive pancreatic enzyme encoded by the CEL gene. Mutations in CEL cause maturity-onset diabetes of the young as well as pancreatic exocrine dysfunction. The enzyme carboxyl ester lipase (CEL), also known as bile salt-dependent or -stimulated lipase (BSDL, BSSL), hydrolyzes dietary fat, cholesteryl esters and fat-soluble vitamins in the duodenum. CEL is mainly expressed in pancreatic acinar cells and lactating mammary glands. The human CEL gene resides on chromosome 9q34.3 and contains a variable number of tandem repeats (VNTR) region that encodes a mucin-like protein tail.

NCBI and Uniprot Product Information

• NCBI GI #: 6753406
• NCBI GeneID: 12613
• NCBI Accession #: NP_034015.1
• NCBI GenBank Nucleotide #: NM_009885.1
• UniProt Accession #: Q64285
• Molecular Weight: 65,813 Da
• NCBI Official Full Name: bile salt-activated lipase
• NCBI Official Synonym Full Names: carboxyl ester lipase
• NCBI Official Symbol: Cel
• NCBI Official Synonym Symbols: BAL; 1810036E18Rik
• NCBI Protein Information: bile salt-activated lipase; BSSL; sterol esterase; cholesterol esterase; bile salt-stimulated lipase; pancreatic lysophospholipase
• UniProt Protein Name: Bile salt-activated lipase
• Protein Family: Cellulase
• UniProt Gene Name: Cel
• UniProt Synonym Gene Names: Lip1; BAL; BSSL
• UniProt Entry Name: CEL_MOUSE

Uniprot Description

CEL: Catalyzes fat and vitamin absorption. Acts in concert with pancreatic lipase and colipase for the complete digestion of dietary triglycerides. Defects in CEL are a cause of maturity-onset diabetes of the young type 8 with exocrine dysfunction (MODY8); also known as diabetes and pancreatic exocrine dysfunction (DPED). MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. Belongs to the type-B carboxylesterase/lipase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Lipid Metabolism - glycerolipid; Lipase; Lipid Metabolism - steroid biosynthesis; Secreted; EC 3.1.1.3; EC 3.1.1.13

Cellular Component: Golgi apparatus; extracellular space; protein complex; rough endoplasmic reticulum; intracellular membrane-bound organelle; cytoplasm; extracellular region; cytosol; lipid raft

Molecular Function: sterol esterase activity; retinyl-palmitate esterase activity; triacylglycerol lipase activity; hydrolase activity; protein complex binding; glycosphingolipid binding; lysophospholipase activity

Biological Process: retinol metabolic process; ceramide catabolic process; lipid metabolic process; pancreatic juice secretion; cholesterol catabolic process; lipid catabolic process