Western Blot (WB)
(SPRED3 Antibody (N-term) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the SPRED3 antibody detected the SPRED3 protein (arrow).)
Rabbit anti-Human, Mouse SPRED3 Polyclonal Antibody | anti-SPRED3 antibody
SPRED3, NT (SPRED3, EVE-3, Sprouty-related, EVH1 domain-containing protein 3) (Azide free) (HRP)
Gene Names
SPRED3; Eve-3; spred-3
Reactivity
Human, Mouse
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
SPRED3; Polyclonal Antibody; NT (SPRED3; EVE-3; Sprouty-related; EVH1 domain-containing protein 3) (Azide free) (HRP); EVH1 domain-containing protein 3; anti-SPRED3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-SPRED3 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
SPRED3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 37-67 amino acids from the N-terminal region of human SPRED3.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(SPRED3 Antibody (N-term) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the SPRED3 antibody detected the SPRED3 protein (arrow).)
Western Blot (WB)
(SPRED3 Antibody (N-term) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the SPRED3 antibody detected the SPRED3 protein (arrow).)
Immunohistochemistry (IHC)
(SPRED3 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of the SPRED3 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(SPRED3 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of the SPRED3 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-SPRED3 antibody
SPRED3 is a member of the Sprouty (see SPRY1; MIM 602465)/SPRED family of proteins that regulate growth factor-induced activation of the MAP kinase cascade (see MAPK1; MIM 176948) (Nonami et al., 2004 [PubMed 15465815]).
Product Categories/Family for anti-SPRED3 antibody
References
King, J.A., et al. J. Hepatol. 44(4):758-767(2006). Nonami, A., et al. J. Biol. Chem. 279(50):52543-52551(2004). Mizoguchi, M., et al. Neurogenetics 5(1):81-82(2004). Kato, R., et al. Biochem. Biophys. Res. Commun. 302(4):767-772(2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,670 Da
NCBI Official Full Name
sprouty-related, EVH1 domain-containing protein 3
NCBI Official Synonym Full Names
sprouty-related, EVH1 domain containing 3
NCBI Official Symbol
SPRED3
NCBI Official Synonym Symbols
Eve-3; spred-3
NCBI Protein Information
sprouty-related, EVH1 domain-containing protein 3
UniProt Protein Name
Sprouty-related, EVH1 domain-containing protein 3
Protein Family
UniProt Gene Name
SPRED3
UniProt Synonym Gene Names
EVE-3; Spred-3
UniProt Entry Name
SPRE3_HUMAN
Uniprot Description
SPRED3: Tyrosine kinase substrate that inhibits growth-factor- mediated activation of MAP kinase. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 19q13
Cellular Component: plasma membrane
Biological Process: multicellular organismal development; inactivation of MAPK activity
Similar Products
Product Notes
The SPRED3 spred3 (Catalog #AAA6350810) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SPRED3, NT (SPRED3, EVE-3, Sprouty-related, EVH1 domain-containing protein 3) (Azide free) (HRP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SPRED3 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-100 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SPRED3 spred3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPRED3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
