Fibronectin, Bovine Active Protein | fn1 active protein

Fibronectin, Bovine

Cat. Num. AAA635038

• Gene Names: fn1; fibronectin
• Applications: ELISA, Western Blot, Gel Super Shift Assay
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Fibronectin; Bovine; fn1 active protein

• Host: Bovine plasma
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a sterile-filtered solution in 0.4M Urea, 50mM Tris-HCl, pH 7.5, 0.5M sodium chloride.
• Concentration: 1.37 mg/ml (varies by lot)

• Applicable Applications for fn1 active protein: ELISA (EL/EIA), Western Blot (WB), Gel Shift Assay (GS/EMSA)
• Application Notes: ELISA, Ouchterlony, Immunoblotting. Used in vitro differentiation of mouse ES cells into neural cells. Suitable for use in mouse embryonic stem cell research. Use in Basal Medium A and as a coating agent for coverslips. This component is recommended for growth and survival of neural cell types during in vitro differentiation of mouse ES cells.
• Activity: Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture.
• Endotoxin Level: 1.0 EU per 1 ug of fibronectin as determined by the LAL method.
• Recommended Dilution: To coat coverslips, prepare a 1mg/ml solution. For use as a substratum for cell attachment and spreading, use at 1-5ug/cm2. To support cell spreading, add 0.5-50ug/ml in media.
• Preparation and Storage: Samples are stable for up to six months at 2-8 degree C. Product is not stable at room temperature. Avoid vortexing and excessive agitation. Aliquot into 50-100ul volumes and store at -20 degree C for up to six months in a manual defrost; freezer. Avoid repeated freeze-thaw cycles.

Related Product Information for fn1 active protein

Fibronectin is an extracellular matrix (ECM) component and is one of the primary cell adhesion molecules.1 It is composed of multiple homologous repeats and contains many functional domains. The occurrence of different isoforms is due to alternative mRNA splicing of the ED-A, ED-B and III-CS regions and subsequent post-translational modification. Although non-reactive with adhesion receptors in its soluble state, fibronectin is highly adhesive when on the surface.2 Polymerization of fibronectin into ECM must be tightly regulated to ensure appropriate adhesive proporties upon ECM formation. Because of its ability to interact with many ligands (e.g. cells, heparin, fibrin, collagen, DNA, immunoglobulin), fibronectin plays an important role in normal

Product Categories/Family for fn1 active protein

Molecular Biology; MB-Fibronectin

NCBI and Uniprot Product Information

• NCBI GI #: 148237139
• NCBI GeneID: 397744
• NCBI Accession #: NP_001081270.1
• NCBI GenBank Nucleotide #: NM_001087801.1
• UniProt Accession #: Q91740
• Molecular Weight: 220kD
• NCBI Official Full Name: fibronectin
• NCBI Official Synonym Full Names: fibronectin 1
• NCBI Official Symbol: fn1
• NCBI Official Synonym Symbols: fibronectin
• NCBI Protein Information: fibronectin; FN; gene C
• UniProt Protein Name: Fibronectin
• Protein Family: Fibronectin
• UniProt Gene Name: fn1
• UniProt Synonym Gene Names: FN
• UniProt Entry Name: FINC_XENLA

Uniprot Description

Function: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. May be involved in osteoblast compaction

By similarity.

Subunit structure: Dimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends

By similarity.

Tissue specificity: In early Xenopus embryo, cellular forms of fibronectin predominate which include both extra domains. In fibronectin of embryonic and adult liver the connecting strand 3 can be either completely excluded or included.

Post-translational modification: Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).

Sequence similarities: Contains 12 fibronectin type-I domains.Contains 2 fibronectin type-II domains.Contains 17 fibronectin type-III domains.