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Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human colon cancer using MBS769354 (FN1 antibody) at dilution of 1:50)

Rabbit Fibronectin Polyclonal Antibody | anti-fn1 antibody

Fibronectin Rabbit Polyclonal

Gene Names
fn1; fibronectin
Reactivity
Human, Mouse, Rat.; other species are not tested.
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
>=95% as determined by SDS-PAGE
Immunogen Affinity Purified
Synonyms
Fibronectin; Polyclonal Antibody; Fibronectin Rabbit Polyclonal; CIG; Cold insoluble globulin; DKFZp686F10164; DKFZp686H0342; DKFZp686I1370; DKFZp686O13149; ED B; fibronectin 1; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF; anti-fn1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat.; other species are not tested.
Clonality
Polyclonal
Isotype
IgG
Specificity
Please decide specifity by homology.
Purity/Purification
>=95% as determined by SDS-PAGE
Immunogen Affinity Purified
Form/Format
Liquid; PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Sequence Length
2481
Applicable Applications for anti-fn1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:200-1:5000
IHC: 1:50-1:200
Immunogen
Fibronectin 1
Calculated MW
260 kDa
Preparation and Storage
Store at -20°C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon cancer using MBS769354 (FN1 antibody) at dilution of 1:50)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human colon cancer using MBS769354 (FN1 antibody) at dilution of 1:50)

Testing Data

()

Testing Data ()

Western Blot (WB)

(U-87MG cells were subjected to SDS PAGE followed by western blot with MBS769354 (FN1 antibody) at dilution of 1:2000)

Western Blot (WB) (U-87MG cells were subjected to SDS PAGE followed by western blot with MBS769354 (FN1 antibody) at dilution of 1:2000)
Related Product Information for anti-fn1 antibody
Fibronectin 1(FN1) is a high molecular weight glycoprotein which exists in both a soluble form in plasma(plasma FN1) and other body fluids and an insoluble form in the extracellular matrix(cellular FN1). Plasma FN1(dimeric form) is secreted by hepatocytes. Cellular FN(dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in theextracellular matrix. FN1 binds to cell surfaces through integrins and to various compoundsincluding collagen, fibrin and heparin. It is involved in cell adhesion and migration processes including embryogenesis, wound healing, hemostasis, host defense, and metastasis.(PMID: 7276612; 12244123; 12847088)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
fibronectin
NCBI Official Synonym Full Names
fibronectin 1
NCBI Official Symbol
fn1
NCBI Official Synonym Symbols
fibronectin
NCBI Protein Information
fibronectin
UniProt Protein Name
Fibronectin
Protein Family
UniProt Gene Name
fn1
UniProt Synonym Gene Names
FN
UniProt Entry Name
FINC_XENLA

Uniprot Description

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. May be involved in osteoblast compaction ().

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Product Notes

The fn1 fn1 (Catalog #AAA769354) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Fibronectin Rabbit Polyclonal reacts with Human, Mouse, Rat.; other species are not tested. and may cross-react with other species as described in the data sheet. AAA Biotech's Fibronectin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). WB: 1:200-1:5000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the fn1 fn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fibronectin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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