Rabbit anti-Human Fibronectin Monoclonal Antibody | anti-FN1 antibody

Recombinant Anti-Fibronectin Antibody, Rabbit Monoclonal

Cat. Num. AAA8102225

• Reactivity: Human
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A
• Synonyms: Fibronectin; Monoclonal Antibody; Recombinant Anti-Fibronectin Antibody; Rabbit Monoclonal; Fibronectin Fragment 2 Antibody; Rabbit MAb; fibronectin 1; Anti-CIG Antibody; Anti-ED-B Antibody; Anti-FINC Antibody; Anti-FN Antibody; Anti-FNZ Antibody; Anti-GFND Antibody; Anti-GFND2 Antibody; Anti-LETS Antibody; Anti-MSF Antibody; anti-FN1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 014
• Specificity: Human Fibronectin Fragment 2
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-FN1 antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IP: 1-4uL/mg of lysate
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Human Fibronectin Fragment 2 protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human Fibronectin Fragment 2.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-FF2 rabbit monoclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:233 kDaObserved band size:250 kDa)

Immunoprecipitation (IP)

(FF2 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate2 uL anti-FF2 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-FF2 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 262 kDaObserved band size: 262 kDa)

Related Product Information for anti-FN1 antibody

Fibronectin (FN) is a glycoprotein component of the extracellular matrix of the extracellular matrix (ECM) with roles in embryogenesis, development, and wound healing. More recently, FN has emerged as player in platelet thrombus formation and diseases associated with thrombosis including vascular remodeling, atherosclerosis, and cardiac repair following a myocardial infarct. Each monomer of FN consists of three types of homologous repeating units, that is 12 type I repeats, two type II repeats and 15-17 type III repeats. The occurrence of multiple isoforms results from alternative mRNA splicing of the ED-A, ED-B and III-CS regions, and subsequent post-translational modification. As an ECM component and one of the primary cell adhesion molecules, Fibronectin can be a ligand for fibrin, heparin, chondroitin sulfate, collagen/gelatin, as well as many integrin receptors through which FN mediates the variety of cellular signaling pathways. The study of solid human tumors showed among the early signs of malignant transformation the fragmentation of pericellular FN, concommitent with the increase of its production by the peritumoral stroma. These results should encourage further investigations concerning the potential importance of Fn production and breakdown during cancer progression. FN1 expression has been described to increase significantly from the morula towards the early blastocyst stage, suggesting that FN1 may also be involved in early blastocyst formation. The fragment 2 of FN comprises the first 7 FN type III repeats and is suggested to be important for self association during fibril growth via the key module III2.

References

Labat-Robert J. (2002) Fibronectin in malignancy. Semin Cancer Biol. 12(3): 187-95.Goossens K, et al. (2009) Quantification of fibronectin 1 (FN1) splice variants, including two novel ones, and analysis of integrins as candidate FN1 receptors in bovine preimplantation embryos. BMC Dev Biol. 9: 1.Maurer LM, et al. (2010) Emerging roles of fibronectin in thrombosis. Thromb Res. 125(4): 287-91.

NCBI and Uniprot Product Information

• Protein Family: Fibronectin