Rabbit anti-Human SOX4 Polyclonal Antibody | anti-SOX4 antibody

SOX4, NT (SOX4, Transcription factor SOX-4) (MaxLight 650)

Cat. Num. AAA6350082

• Gene Names: SOX4; EVI16
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: SOX4; Polyclonal Antibody; NT (SOX4; Transcription factor SOX-4) (MaxLight 650); Transcription factor SOX-4; anti-SOX4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-SOX4 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: SOX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93~122 amino acids from the N-terminal region of human SOX4.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of SOX4 antibody (N-term) in A2058 cell line lysates (35ug/lane). SOX4 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain reacted with SOX4 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(SOX4 Antibody (N-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Fluorescent confocal image of Hela cell stained with SOX4 Antibody (N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SOX4 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). SOX4 immunoreactivity is localized to Mitochondria significantly and Nucleus weakly. )

Related Product Information for anti-SOX4 antibody

SOX4 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The protein may act as a transcriptional regulator after forming a protein complex with other proteins, such as syndecan binding protein (syntenin). The protein may function in the apoptosis pathway leading to cell death as well as to tumorigenesis and may mediate downstream effects of parathyroid hormone (PTH) and PTH-related protein (PTHrP) in bone development. The solution structure has been resolved for the HMG-box of a similar mouse protein.

Product Categories/Family for anti-SOX4 antibody

Antibodies; Transcription Factors

References

Farr C.J., Easty D.J.Mamm. Genome 4:577-584(1993) Pan X., Li H., Zhang P.Biochem. Biophys. Res. Commun. 344:727-734(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 4507163
• NCBI GeneID: 6659
• NCBI Accession #: NP_003098
• NCBI GenBank Nucleotide #: NM_003107.2
• UniProt Accession #: Q06945
• Molecular Weight: Predicted: 52 kDa; ; Observed: 520 kDa
• NCBI Official Full Name: transcription factor SOX-4
• NCBI Official Synonym Full Names: SRY (sex determining region Y)-box 4
• NCBI Official Symbol: SOX4
• NCBI Official Synonym Symbols: EVI16
• NCBI Protein Information: transcription factor SOX-4; SRY-related HMG-box gene 4; ecotropic viral integration site 16
• UniProt Protein Name: Transcription factor SOX-4
• Protein Family: Transcription factor
• UniProt Gene Name: SOX4
• UniProt Entry Name: SOX4_HUMAN

NCBI Description

This intronless gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins, such as syndecan binding protein (syntenin). The protein may function in the apoptosis pathway leading to cell death as well as to tumorigenesis and may mediate downstream effects of parathyroid hormone (PTH) and PTH-related protein (PTHrP) in bone development. The solution structure has been resolved for the HMG-box of a similar mouse protein. [provided by RefSeq, Jul 2008]

Uniprot Description

SOX4: Transcriptional activator that binds with high affinity to the T-cell enhancer motif 5'-AACAAAG-3' motif.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleus

Molecular Function: protein binding; protein heterodimerization activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; negative regulation of protein export from nucleus; positive regulation of translation; somatic stem cell maintenance; positive regulation of apoptosis; heart development; positive regulation of transcription, DNA-dependent; regulation of protein stability; Wnt receptor signaling pathway through beta-catenin; glucose homeostasis; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; glial cell proliferation; negative regulation of cell proliferation; sympathetic nervous system development; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; positive regulation of Wnt receptor signaling pathway; skeletal development; T cell differentiation; glial cell development; pro-B cell differentiation; protein stabilization; positive regulation of insulin secretion; DNA damage response, detection of DNA damage; spinal cord motor neuron differentiation; endocrine pancreas development; limb bud formation; spinal cord development; negative regulation of protein ubiquitination; positive regulation of transcription from RNA polymerase II promoter; neural tube formation