Western Blot (WB)
(Western blot analysis of SOX4 antibody (N-term) in A2058 cell line lysates (35ug/lane). SOX4 (arrow) was detected using the purified Pab.)
Rabbit anti-Human SOX4 Polyclonal Antibody | anti-SOX4 antibody
SOX4, NT (SOX4, Transcription factor SOX-4) (MaxLight 490)
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:50-100
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western blot analysis of SOX4 antibody (N-term) in A2058 cell line lysates (35ug/lane). SOX4 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of SOX4 antibody (N-term) in A2058 cell line lysates (35ug/lane). SOX4 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain reacted with SOX4 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain reacted with SOX4 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(SOX4 Antibody (N-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(SOX4 Antibody (N-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with SOX4 Antibody (N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SOX4 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). SOX4 immunoreactivity is localized to Mitochondria significantly and Nucleus weakly. )
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with SOX4 Antibody (N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SOX4 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). SOX4 immunoreactivity is localized to Mitochondria significantly and Nucleus weakly. )
NCBI and Uniprot Product Information
Observed: 520 kDa
NCBI Description
This intronless gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins, such as syndecan binding protein (syntenin). The protein may function in the apoptosis pathway leading to cell death as well as to tumorigenesis and may mediate downstream effects of parathyroid hormone (PTH) and PTH-related protein (PTHrP) in bone development. The solution structure has been resolved for the HMG-box of a similar mouse protein. [provided by RefSeq, Jul 2008]
Uniprot Description
SOX4: Transcriptional activator that binds with high affinity to the T-cell enhancer motif 5'-AACAAAG-3' motif.
Protein type: Transcription factor; DNA-binding
Chromosomal Location of Human Ortholog: 6p22.3
Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleus
Molecular Function: protein binding; protein heterodimerization activity; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; negative regulation of protein export from nucleus; positive regulation of translation; somatic stem cell maintenance; positive regulation of apoptosis; heart development; positive regulation of transcription, DNA-dependent; regulation of protein stability; Wnt receptor signaling pathway through beta-catenin; glucose homeostasis; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; glial cell proliferation; negative regulation of cell proliferation; sympathetic nervous system development; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; positive regulation of Wnt receptor signaling pathway; skeletal development; T cell differentiation; glial cell development; pro-B cell differentiation; protein stabilization; positive regulation of insulin secretion; DNA damage response, detection of DNA damage; spinal cord motor neuron differentiation; endocrine pancreas development; limb bud formation; spinal cord development; negative regulation of protein ubiquitination; positive regulation of transcription from RNA polymerase II promoter; neural tube formation
Research Articles on SOX4
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Product Notes
The SOX4 sox4 (Catalog #AAA6350080) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SOX4, NT (SOX4, Transcription factor SOX-4) (MaxLight 490) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SOX4 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IHC: 1:50-100 WB: 1:50-100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SOX4 sox4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SOX4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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