SMOC1 active protein

SMOC1 (SPARC-related Modular Calcium-binding Protein 1, Secreted Modular Calcium-binding Protein 1, SMOC-1)

Cat. Num. AAA634998

• Gene Names: SMOC1; OAS
• Purity: Highly Purified; ~95% (SDS-PAGE, reducing conditions)
• Synonyms: SMOC1; SMOC1 (SPARC-related Modular Calcium-binding Protein 1; Secreted Modular Calcium-binding Protein 1; SMOC-1); SMOC1 active protein

• Purity/Purification: Highly Purified; ~95% (SDS-PAGE, reducing conditions)
• Form/Format: Supplied as a lyophilized powder from PBS. Reconstitute with sterile PBS.

• Application Notes: SDS-PAGE: 60-65kD, reducing conditions
• Activity: Measured by the ability of the immobilized protein to support the adhesion of A431 human epidermoid carcinoma cells. When 5x10e4 cells/well are added to rhSMOC-1 coated plates, cell adhesion is enhanced in a dose dependent manner after 75 minutes at 37 degree C. The ED50 for this effect is typically 0.5-2ug/ml.
• Endotoxin Level: 1EU/1ug (LAL)
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for SMOC1 active protein

SMOC-1 (secreted, or SPARC-related, modular calcium-binding protein 1) is a 70-90kD secreted glycoprotein that is a member of the SPARC family of matricellular molecules. Mature human SMOC-1 is 408aa in length. It contains one Kazal-like domain (aa42-87), two thyroglobulin type-1 (TY) segments (aa92-159 and 224-292) and two EF-hand sequences (aa359-394 and 396-431). One potential splice variant contains a 16aa region of alternate sequence between the TY domains, a 7aa deletion between the TY and EF-hand domains, and three alternate aa at the C-terminus. Mature human SMOC-1 shares 92% aa identity with mouse and rat SMOC-1 and 97%, 96% and 95% aa identity with canine, bovine and porcine SMOC-1, respectively. The principal difference between rodents and other mammals is an additional 19aa near the C-terminus of rodent SMOC-1. Like other matricellular proteins, SMOC-1 is primarily expressed in basement membranes, although it has also been found in other extracellular matrices and the oocyte zona pellucida. It is present early in mouse embryogenesis, and is produced by cells deriving from all three germ layers. Recombinant bacterially produced human SMOC-1 and SMOC-2 were both shown to bind the acute phase protein, C-reactive protein, and the adhesion proteins, fibulin and vitronectin. A signaling role for SMOC-1 was shown in rat mesangial cells: induction of nitric oxide in response to inflammatory cytokines downregulates SMOC-1 which, in turn, downregulates expression of TGF-b and TGF-b-regulated genes. This mechanism is proposed to limit the profibrotic effects of TGF-b, for example in the glomerulus. In Xenopus, the SMOC paralog has been shown to antagonize BMP activity.

Product Categories/Family for SMOC1 active protein

Molecular Biology; MB-Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 78190498
• NCBI GeneID: 64093
• NCBI Accession #: NP_001030024.1
• NCBI GenBank Nucleotide #: NM_001034852.2
• UniProt Accession #: Q9H4F8; Q96F78; A8K1S3; B2R7P5
• Molecular Weight: ~46kD
• NCBI Official Full Name: SPARC-related modular calcium-binding protein 1 isoform 1
• NCBI Official Synonym Full Names: SPARC related modular calcium binding 1
• NCBI Official Symbol: SMOC1
• NCBI Official Synonym Symbols: OAS
• NCBI Protein Information: SPARC-related modular calcium-binding protein 1; secreted modular calcium-binding protein 1
• UniProt Protein Name: SPARC-related modular calcium-binding protein 1
• Protein Family: SPARC-related modular calcium-binding protein
• UniProt Gene Name: SMOC1
• UniProt Synonym Gene Names: SMOC-1
• UniProt Entry Name: SMOC1_HUMAN

NCBI Description

This gene encodes a multi-domain secreted protein that may have a critical role in ocular and limb development. Mutations in this gene are associated with microphthalmia and limb anomalies. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]

Uniprot Description

SMOC1: Plays essential roles in both eye and limb development. Probale regulator of osteoblast differentiation. Defects in SMOC1 are the cause of ophthalmoacromelic syndrome (OAS). A rare disorder presenting with anophthalmia or microphthalmia and limb anomalies. Limb malformations include fused 4th and 5th metacarpals and short 5th finger in hands, and olygodactyly in foot (four toes). Most patients have bilateral anophthalmia/ microphthalmia, but unilateral abnormality is also noted. Other malformations are rare, but venous or vertebral anomaly was recognized each in single cases. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 14q24.2

Cellular Component: basement membrane

Molecular Function: protein binding; extracellular matrix binding; calcium ion binding

Biological Process: limb development; extracellular matrix organization and biogenesis; regulation of osteoblast differentiation; eye development; signal transduction; cell differentiation

Disease: Microphthalmia With Limb Anomalies