Rabbit anti-Human RSPO4 Polyclonal Antibody | anti-RSPO4 antibody

RSPO4, CT (RSPO4, C20orf182, R-spondin-4, Roof plate-specific spondin-4) (AP)

Cat. Num. AAA6344227

• Gene Names: RSPO4; CRISTIN4; C20orf182
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RSPO4; Polyclonal Antibody; CT (RSPO4; C20orf182; R-spondin-4; Roof plate-specific spondin-4) (AP); Roof plate-specific spondin-4; anti-RSPO4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-RSPO4 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: RSPO4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 206-236 amino acids from the C-terminal region of human RSPO4.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(RSPO4 Antibody (C-term) western blot analysis in Jurkat cell line lysates (15ug/lane).This demonstrates the RSPO4 antibody detected the RSPO4 protein (arrow).)

Immunohistochemistry (IHC)

(RSPO4 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RSPO4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(RSPO4 Antibody (C-term) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-RSPO4 antibody

RSPO4 is a member of the R-spondin family of proteins that share a common domain organization consisting of a signal peptide, cysteine-rich/furin-like domain, thrombospondin domain and a C-terminal basic region. The encoded protein may be involved in activation of Wnt/beta-catenin signaling pathways. Mutations in this gene are associated with anonychia congenital. Alternate splicing results in multiple transcript variants.

Product Categories/Family for anti-RSPO4 antibody

Antibodies; KIAA; orf

References

Bergmann, C., et al. Am. J. Hum. Genet. 79(6):1105-1109(2006). Blaydon, D.C., et al. Nat. Genet. 38(11):1245-1247(2006). Kim, K.A., et al. Cell Cycle 5(1):23-26(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 83722284
• NCBI GeneID: 343637
• NCBI Accession #: NP_001025042.2
• NCBI GenBank Nucleotide #: NM_001029871.3
• UniProt Accession #: Q2I0M5; Q9UGB2; A2A2I6
• Molecular Weight: 19,607 Da
• NCBI Official Full Name: R-spondin-4 isoform 1
• NCBI Official Synonym Full Names: R-spondin 4
• NCBI Official Symbol: RSPO4
• NCBI Official Synonym Symbols: CRISTIN4; C20orf182
• NCBI Protein Information: R-spondin-4
• UniProt Protein Name: R-spondin-4
• Protein Family: R-spondin
• UniProt Gene Name: RSPO4
• UniProt Synonym Gene Names: C20orf182; hRspo4
• UniProt Entry Name: RSPO4_HUMAN

NCBI Description

This gene encodes a member of the R-spondin family of proteins that share a common domain organization consisting of a signal peptide, cysteine-rich/furin-like domain, thrombospondin domain and a C-terminal basic region. The encoded protein may be involved in activation of Wnt/beta-catenin signaling pathways. Mutations in this gene are associated with anonychia congenital. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2009]

Uniprot Description

R-spondin-4: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Defects in RSPO4 are the cause of nail disorder non- syndromic congenital type 4 (NDNC4). A nail disorder characterized by congenital anonychia or its milder phenotypic variant hyponychia. Anonychia/hyponychia is the absence or severe hypoplasia of all fingernails and toenails without significant bone anomalies. Belongs to the R-spondin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Cell development/differentiation

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: extracellular region

Disease: Nail Disorder, Nonsyndromic Congenital, 4