Rabbit anti-Human RSPO1 Polyclonal Antibody | anti-RSPO1 antibody

RSPO1, NT (R-spondin-1, Roof Plate-specific Spondin-1, hRspo1)

Cat. Num. AAA6005248

• Gene Names: RSPO1; RSPO; CRISTIN3; RP11-566C13.1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: RSPO1; Polyclonal Antibody; NT (R-spondin-1; Roof Plate-specific Spondin-1; hRspo1); Anti -RSPO1; anti-RSPO1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human RSPO1. Species sequence homology: mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-RSPO1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1-2ug/ml; Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
• Immunogen: Synthetic peptide corresponding to 16aa from human RSPO1 at N-terminus.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry (IHC)

(Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(Western blot analysis of RSPO1 in rat spleen tissue lysate with RSPO1 antibody at (A) 1 and (B) 2ug/ml.)

Related Product Information for anti-RSPO1 antibody

R-Spondin 1 (RSPO1, Roof plate-specific Spondin 1), also known as cysteine-rich and single thrombospondin domain containing protein 3 (Cristin 3), is a 27kD secreted protein that shares 40% aa identity with three otherR-Spondin family members. All R-Spondins regulate Wnt/b-catenin signaling, but have distinct expression patterns. Like other R-Spondins, R-Spondin 1 contains two adjacent cysteine-rich furin-like domains (aa34-135) with one potential N-glycosylation site, followed by a thrombospondin (TSP-1) motif (aa147-207) and a region rich in basic residues (aa211-263). Only the furin-like domains are needed for b-catenin stabilization. A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus. Potential isoforms of 200-236aa have an alternate, shorter N-terminus or are missing aa146-208, respectively. Over aa21-263, human R-Spondin 1 shares 89%, 87%, 92%, 91%, 91% and 89aa identity with mouse, rat, equine, canine, caprine and bovine R-Spondin 1, respectively. R-Spondin 1 is expressed in early development at the roof plate boundary and is thought to contribute to dorsal neural tube development. In humans, rare disruptions of the R-Spondin 1 gene are associated with tendencies for XX sex reversal (phenotypic male) or hermaphroditism, indicating a role for R-Spondin 1 in gender-specific differentiation. Disruption is also associated with palmoplantar keratosis. Postnatally, R-Spondin 1 is expressed by neuroendocrine cells in the intestine, adrenal gland and pancreas, and by epithelia in kidney and prostate. Injection of recombinant R-Spondin 1 in mice causes activation of b-catenin and proliferation of intestinal crypt epithelial cells, and ameliorates experimental colitis. R-Spondin 1 regulates Wnt/b-catenin by competing with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors, Kremen and LRP-6, reducing their DKK-1-mediated internalization. Reports differ on whether R-Spondin 1 binds LRP-6 directly.

Product Categories/Family for anti-RSPO1 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 339276103
• NCBI GeneID: 284654
• NCBI Accession #: NP_001229839.1
• NCBI GenBank Nucleotide #: NM_001242910.1
• UniProt Accession #: Q2MKA7; Q0H8S6; Q14C72; Q5T0F2; Q8N7L5; A2A420
• Molecular Weight: 28,959 Da
• NCBI Official Full Name: R-spondin-1 isoform 3
• NCBI Official Synonym Full Names: R-spondin 1
• NCBI Official Symbol: RSPO1
• NCBI Official Synonym Symbols: RSPO; CRISTIN3; RP11-566C13.1
• NCBI Protein Information: R-spondin-1; R-spondin homolog; roof plate-specific spondin-1
• UniProt Protein Name: R-spondin-1
• Protein Family: R-spondin
• UniProt Gene Name: RSPO1
• UniProt Synonym Gene Names: hRspo1
• UniProt Entry Name: RSPO1_HUMAN

NCBI Description

This gene is a member of the R-spondin family and encodes a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. [provided by RefSeq, Jul 2008]

Uniprot Description

R-spondin-1: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. Defects in RSPO1 are the cause of palmoplantar keratoderma with squamous cell carcinoma of skin and sex reversal (PKKSCC). This recessive syndrome is characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. Belongs to the R-spondin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Secreted; Secreted, signal peptide; Cell adhesion

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: extracellular space; extracellular region; nucleus

Molecular Function: heparin binding; protein binding; G-protein-coupled receptor binding; receptor binding

Biological Process: regulation of gene expression; regulation of receptor internalization; Wnt receptor signaling pathway through beta-catenin; positive regulation of protein amino acid phosphorylation; male meiosis; positive regulation of Wnt receptor signaling pathway

Disease: Palmoplantar Hyperkeratosis With Squamous Cell Carcinoma Of Skin And 46,xx Sex Reversal