Rabbit anti-Human RSPO4 Polyclonal Antibody | anti-RSPO4 antibody

RSPO4 Antibody (C-term)

Cat. Num. AAA9203044

• Gene Names: RSPO4; CRISTIN4; C20orf182
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: RSPO4; Polyclonal Antibody; RSPO4 Antibody (C-term); R-spondin-4; Roof plate-specific spondin-4; hRspo4; C20orf182; anti-RSPO4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This RSPO4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 199-228 amino acids from the C-terminal region of human RSPO4.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 199-228
• Sequence Length: 172

• Applicable Applications for anti-RSPO4 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry Paraffin (IHC-P), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000; IHC-P~~1:50~100; FC~~1:10~50
• Function: Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors (PubMed:29769720). Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway (PubMed:21727895, PubMed:21909076).
• Cellular Location: Secreted.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(RSPO4 Antibody (C-term) (MBS9203044) western blot analysis in Jurkat cell line lysates (15ug/lane).This demonstrates the RSPO4 antibody detected the RSPO4 protein (arrow).)

Immunohistochemistry (IHC)

(RSPO4 Antibody (C-term) (MBS9203044) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RSPO4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(RSPO4 Antibody (C-term)(MBS9203044) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-RSPO4 antibody

RSPO4 is a member of the R-spondin family of
proteins that share a common domain organization consisting of a
signal peptide, cysteine-rich/furin-like domain, thrombospondin
domain and a C-terminal basic region. The encoded protein may be
involved in activation of Wnt/beta-catenin signaling pathways.
Mutations in this gene are associated with anonychia congenital.
Alternate splicing results in multiple transcript variants.

References

Bergmann, C., et al. Am. J. Hum. Genet. 79(6):1105-1109(2006)
Blaydon, D.C., et al. Nat. Genet. 38(11):1245-1247(2006)
Kim, K.A., et al. Cell Cycle 5(1):23-26(2006)

NCBI and Uniprot Product Information

• NCBI GI #: 83722284
• NCBI GeneID: 343637
• NCBI Accession #: NP_001025042.2; NP_001035096.1
• NCBI GenBank Nucleotide #: NM_001029871.3
• UniProt Accession #: Q2I0M5; Q9UGB2; A2A2I6
• NCBI Official Full Name: R-spondin-4 isoform 1
• NCBI Official Synonym Full Names: R-spondin 4
• NCBI Official Symbol: RSPO4
• NCBI Official Synonym Symbols: CRISTIN4; C20orf182
• NCBI Protein Information: R-spondin-4
• UniProt Protein Name: R-spondin-4
• Protein Family: R-spondin
• UniProt Gene Name: RSPO4
• UniProt Synonym Gene Names: C20orf182; hRspo4
• UniProt Entry Name: RSPO4_HUMAN

NCBI Description

This gene encodes a member of the R-spondin family of proteins that share a common domain organization consisting of a signal peptide, cysteine-rich/furin-like domain, thrombospondin domain and a C-terminal basic region. The encoded protein may be involved in activation of Wnt/beta-catenin signaling pathways. Mutations in this gene are associated with anonychia congenital. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2009]

Uniprot Description

R-spondin-4: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Defects in RSPO4 are the cause of nail disorder non- syndromic congenital type 4 (NDNC4). A nail disorder characterized by congenital anonychia or its milder phenotypic variant hyponychia. Anonychia/hyponychia is the absence or severe hypoplasia of all fingernails and toenails without significant bone anomalies. Belongs to the R-spondin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Cell development/differentiation; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; G-protein-coupled receptor binding

Biological Process: Wnt receptor signaling pathway

Disease: Nail Disorder, Nonsyndromic Congenital, 4