Rabbit anti-Human RSPO2 Polyclonal Antibody | anti-RSPO2 antibody

RSPO2, CT (RSPO2, R-spondin-2, Roof plate-specific spondin-2) (MaxLight 405)

Cat. Num. AAA6344221

• Gene Names: RSPO2; CRISTIN2
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: RSPO2; Polyclonal Antibody; CT (RSPO2; R-spondin-2; Roof plate-specific spondin-2) (MaxLight 405); Roof plate-specific spondin-2; anti-RSPO2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RSPO2.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-RSPO2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 190-218 in the C-terminal region of human RSPO2, UniProt Accession #Q6UXX9; Q8BFU0. Species sequence homology: mouse
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of lysate from 293 cell line (35ug) using MBS643902 (1:1000). A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma using MBS643902 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS643902 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis with 293 cells using MBS643902 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-RSPO2 antibody

R-spondins (RSPOs), such as RSPO2, are secreted proteins that regulate beta-catenin (CTNNB1; MIM 116806) signaling.

Product Categories/Family for anti-RSPO2 antibody

Antibodies; Proteins

References

1. Kim, K.A., et al. Cell Cycle 5 (1): 23-26 (2006). 2. Kazanskaya, O., et al. Dev. Cell 7 (4):5 25-534 (2004). 3. Clark, H.F., et al. Genome Res. 13(10): 2265-2270 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 222446611
• NCBI GeneID: 340419
• NCBI Accession #: NP_848660.3
• NCBI GenBank Nucleotide #: NM_178565.4
• UniProt Accession #: Q6UXX9; Q4G0U4; Q8N6X6; B3KVP0
• Molecular Weight: 22.0kD
• NCBI Official Full Name: R-spondin-2 isoform 1
• NCBI Official Synonym Full Names: R-spondin 2
• NCBI Official Symbol: RSPO2
• NCBI Official Synonym Symbols: CRISTIN2
• NCBI Protein Information: R-spondin-2; R-spondin 2 homolog; roof plate-specific spondin-2
• UniProt Protein Name: R-spondin-2
• Protein Family: R-spondin
• UniProt Gene Name: RSPO2
• UniProt Synonym Gene Names: hRspo2
• UniProt Entry Name: RSPO2_HUMAN

NCBI Description

R-spondins (RSPOs), such as RSPO2, are secreted proteins that regulate beta-catenin (CTNNB1; MIM 116806) signaling (Kim et al., 2006 [PubMed 16357527]).[supplied by OMIM, Mar 2008]

Uniprot Description

R-spondin-2: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Probably also acts as a ligand for frizzled and LRP receptors. Belongs to the R-spondin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Cell development/differentiation; Secreted

Chromosomal Location of Human Ortholog: 8q23.1

Cellular Component: extracellular space; cell surface; extracellular region

Molecular Function: heparin binding; G-protein-coupled receptor binding; receptor binding

Biological Process: embryonic forelimb morphogenesis; osteoblast differentiation; Wnt receptor signaling pathway; embryonic hindlimb morphogenesis; bone mineralization