Rabbit anti-Human MPP1 Polyclonal Antibody | anti-MPP1 antibody

MPP1, ID (MPP1, DXS552E, EMP55, 55kD erythrocyte membrane protein, Membrane protein, palmitoylated 1) (AP)

Cat. Num. AAA6321922

• Gene Names: MPP1; MRG1; PEMP; AAG12; EMP55; DXS552E
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MPP1; Polyclonal Antibody; ID (MPP1; DXS552E; EMP55; 55kD erythrocyte membrane protein; Membrane protein; palmitoylated 1) (AP); palmitoylated 1; anti-MPP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 466

• Applicable Applications for anti-MPP1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: MPP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-331 amino acids from the Central region of human MPP1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(MPP1 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the MPP1 antibody detected the MPP1 protein (arrow).)

Western Blot (WB)

(Western blot analysis of MPP1 (arrow) using rabbit polyclonal MPP1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MPP1 gene.)

Immunohistochemistry (IHC)

(MPP1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MPP1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(MPP1 Antibody (Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-MPP1 antibody

This gene encodes the prototype of the membrane-associated guanylate kinase (MAGUK) family proteins. MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intercellular junctions. The encoded protein is an extensively palmitoylated membrane phosphoprotein containing a PDZ domain, a Src homology 3 (SH3) motif, and a guanylate kinase domain. This gene product interacts with various cytoskeletal proteins and cell junctional proteins in different tissue and cell types, and may be involved in the regulation of cell shape, hair cell development, neural patterning of the retina, and apico-basal polarity and tumor suppression pathways in non-erythroid cells. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-MPP1 antibody

Antibodies; Membrane Proteins

References

Seo, P.S., et al. Exp. Biol. Med. (Maywood) 234(3):255-262(2009). Seo, P.S., et al. Biochim. Biophys. Acta 1793(2):281-289(2009). Gosens, I., et al. Hum. Mol. Genet. 16(16):1993-2003(2007). Kusunoki, H., et al. Proteins 64(3):804-807(2006). Funke, L., et al. Annu. Rev. Biochem. 74, 219-245 (2005).

NCBI and Uniprot Product Information

• NCBI GI #: 4505237
• NCBI GeneID: 4354
• NCBI Accession #: NP_002427.1
• NCBI GenBank Nucleotide #: NP_002427.1
• UniProt Accession #: Q00013
• NCBI Official Full Name: 55 kDa erythrocyte membrane protein isoform 1
• NCBI Official Synonym Full Names: membrane palmitoylated protein 1
• NCBI Official Symbol: MPP1
• NCBI Official Synonym Symbols: MRG1; PEMP; AAG12; EMP55; DXS552E
• NCBI Protein Information: 55 kDa erythrocyte membrane protein
• UniProt Protein Name: 55 kDa erythrocyte membrane protein
• Protein Family: Mitochondrial-processing peptidase
• UniProt Gene Name: MPP1
• UniProt Synonym Gene Names: DXS552E; EMP55; p55
• UniProt Entry Name: EM55_HUMAN

NCBI Description

This gene encodes the prototype of the membrane-associated guanylate kinase (MAGUK) family proteins. MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intercellular junctions. The encoded protein is an extensively palmitoylated membrane phosphoprotein containing a PDZ domain, a Src homology 3 (SH3) motif, and a guanylate kinase domain. This gene product interacts with various cytoskeletal proteins and cell junctional proteins in different tissue and cell types, and may be involved in the regulation of cell shape, hair cell development, neural patterning of the retina, and apico-basal polarity and tumor suppression pathways in non-erythroid cells. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]