Goat Bacillus anthracis PA, Protective Antigen Polyclonal Antibody
Bacillus anthracis PA, Protective Antigen (Anthrax) (Biotin)
Applications
ELISA, Western Blot
Purity
PurifiedPurified.
Synonyms
Bacillus anthracis PA; Protective Antigen; Polyclonal Antibody; Protective Antigen (Anthrax) (Biotin); Anti -Bacillus anthracis PA; anti-Bacillus anthracis PA; Protective Antigen antibody
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes bothPA63 and PA83 of B. anthracis Lethal Factor. No cross-reactivity with Lethal Factor (LF) of Bacillus anthracis, Y. Pestis, F. Tularensis and Toxoplasma gondi.
Purity/Purification
Purified
Purified.
Purified.
Form/Format
Supplied as a liquid in PBS, pH7.4, 0.1% BSA, 0.1% Proclin-300. Labeled with Biotin.
Applicable Applications for anti-Bacillus anthracis PA, Protective Antigen antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1-10ug/ml (ECL)
Dilution: Western Blot: 1-10ug/ml (ECL)
Immunogen
Recombinant corresponding to purified Bacillus anthracis Protective Antigen (PA83).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-Bacillus anthracis PA, Protective Antigen antibody
After inhalation by mammals, Bacillus anthracis spores germinate in alveolar macrophages then migrate to lymph nodes where they multiply. The vegetative bacteria excrete the tripartite exotoxin which consists of three polypeptides: protective antigen (PA, 83kD), lethal factor (LF, 90kD) and oedma factor (OF, 89kD). The two components (OF and LF) of the toxin enzymatically modify substrates within the cytosol of the mammalian cells: The OF is an adenylate cyclase that impairs the host defenses through a variety of mechanisms inhibiting phagocytosis. The LF is a zinc dependent protease that cleaves several mitogen activated protein kinase kinases (MAPKK) and causes lysis of macrophages. To intoxicate mammalian cells, the third component of the toxin PA, binds to a ubiquitously expressed cellular receptor, Tumor Endothelium Marker-8 (TEM8). Upon binding to TEM8, PA is cleaved into 20 and 63kD fragments (PA20 and PA63) by furin or furin-like proteases. PA20 dissociates into medium and allows the PA63 fragment to heptamerize and bind LF and OF of the toxin. The resulting complex of PA63 fragment with EF and/or OF binds to PA-receptor TEM8/ATR and internalized into endosomes followed by translocation of LF and OF into cytosol of the cells.
Product Categories/Family for anti-Bacillus anthracis PA, Protective Antigen antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
Bacillus anthracis protective antigen
Similar Products
Product Notes
The Bacillus anthracis PA, Protective Antigen (Catalog #AAA630452) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Bacillus anthracis PA, Protective Antigen can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: Western Blot: 1-10ug/ml (ECL). Researchers should empirically determine the suitability of the Bacillus anthracis PA, Protective Antigen for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bacillus anthracis PA, Protective Antigen, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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