Rabbit TBX5 Polyclonal Antibody | anti-TBX5 antibody

TBX5 Antibody

Cat. Num. AAA9612464

• Gene Names: TBX5; HOS
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(80%), Dog(100%), Chicken(100%), Xenopus(100%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: TBX5; Polyclonal Antibody; TBX5 Antibody; Holt Oram syndrome; HOS; T box 5; T box protein 5; T box transcription factor TBX 5; T box transcription factor TBX5; T-box protein 5; T-box transcription factor TBX5; TBX 5; TBX5_HUMAN; Transcription factor T box 5; anti-TBX5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(80%), Dog(100%), Chicken(100%), Xenopus(100%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TBX5 Antibody detects endogenous levels of total TBX5.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 518

• Applicable Applications for anti-TBX5 antibody: ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human TBX5, corresponding to a region within the internal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Hela cells, using TBX5 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-TBX5 antibody

Function: DNA-binding protein that regulates the transcription of several genes and is involved in heart development and limb pattern formation (PubMed:25725155, PubMed:25963046, PubMed:29174768, PubMed:26917986, PubMed:27035640, PubMed:8988164). Binds to the core DNA motif of NPPA promoter (PubMed:26926761).

Post Translational Modifications: Acetylation at Lys-339 by KAT2A and KAT2B promotes nuclear retention.

Subcellular Location: Nucleus. Cytoplasm. Note: Shuttles between the cytoplasm and the nucleus. Acetylation at Lys-339 promotes nuclear retention.

Subunit Structure: Monomer (PubMed:20450920). Homodimer (via the T-box); binds DNA as homodimer (PubMed:26926761). Interacts (via the T-box) with NKX2-5 (via the homeobox); this complex binds DNA (PubMed:26926761). Interacts with GATA4 (PubMed:24000169). Interacts with KAT2A and KAT2B (PubMed:29174768).

Similarity: The T-Box domain binds to double-stranded DNA (PubMed:26926761).

NCBI and Uniprot Product Information

• NCBI GI #: 12644474
• NCBI GeneID: 6910
• UniProt Accession #: Q99593
• Molecular Weight: Observed Molecular Weight: (Observed)57kD.; Predicted Molecular Weight: (Calculated)58kDa.
• NCBI Official Full Name: T-box transcription factor TBX5
• NCBI Official Synonym Full Names: T-box transcription factor 5
• NCBI Official Symbol: TBX5
• NCBI Official Synonym Symbols: HOS
• NCBI Protein Information: T-box transcription factor TBX5
• UniProt Protein Name: T-box transcription factor TBX5
• Protein Family: T-box transcription factor
• UniProt Gene Name: TBX5
• UniProt Synonym Gene Names: T-box protein 5
• UniProt Entry Name: TBX5_HUMAN

NCBI Description

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in this gene have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TBX5: Involved in the transcriptional regulation of genes required for mesoderm differentiation. Probably plays a role in limb pattern formation. Defects in TBX5 are the cause of Holt-Oram syndrome (HOS). HOS is a developmental disorder affecting the heart and upper limbs. It is characterized by thumb anomaly and atrial septal defects. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 12q24.1

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding

Biological Process: embryonic forelimb morphogenesis; pericardium development; transcription initiation from RNA polymerase II promoter; heart development; positive regulation of transcription, DNA-dependent; pattern specification process; cardiac muscle cell differentiation; forelimb morphogenesis; negative regulation of cell proliferation; cell-cell signaling; apoptotic nuclear changes; negative regulation of cardiac muscle cell proliferation; positive regulation of cardioblast differentiation; morphogenesis of an epithelium; gene expression; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell migration; lung development; embryonic limb morphogenesis; positive regulation of cardiac muscle cell proliferation

Disease: Holt-oram Syndrome