Rabbit Caspase 12, IN Polyclonal Antibody | anti-Casp12 antibody

Caspase 12, IN

Cat. Num. AAA629331

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Caspase 12; IN; Polyclonal Antibody; Anti -Caspase 12; anti-Casp12 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse Caspase-12 (IN). Species Crossreactivity: human and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

• Applicable Applications for anti-Casp12 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Immunohistochemisty and Western Blot.; Dilution: Western Blot: 0.5-1ug/ml.; Immunohistochemistry: 2ug/ml for mouse liver tissue.
• Immunogen: Synthetic peptide corresponding to aa (100-116) (CTPSSPSESKRKVEDDE) of murine caspase-12 (3).
• Positive Control: Mouse brain tissue lysate.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile (40-50%) glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of Caspase-12 in (A) human spleen and (B) mouse spleen tissue lysate with Caspase-12 at 1ug/mL.)

Immunohistochemistry (IHC)

(Immunohistochemical staining of mouse liver tissue using MBS629331 at 2ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of Caspase-12 in Mouse Liver cells with Caspase-12 at 10ug/mL.)

Related Product Information for anti-Casp12 antibody

Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the mains, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved (1,2). Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including g-secretase presenilin and b-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-b. Caspase-12 is ubiquitously expressed in mouse tissues (1).

Product Categories/Family for anti-Casp12 antibody

Antibodies; Abs to Apoptosis; Caspase

NCBI and Uniprot Product Information

• NCBI GI #: 18426852
• NCBI GeneID: 156117
• NCBI Accession #: NP_569106.1
• NCBI GenBank Nucleotide #: NM_130422.1
• UniProt Accession #: Q920D5
• Molecular Weight: 47,837 Da
• NCBI Official Full Name: caspase-12
• NCBI Official Synonym Full Names: caspase 12
• NCBI Official Symbol: Casp12
• NCBI Protein Information: caspase-12; CASP-12
• UniProt Protein Name: Caspase-12
• Protein Family: Inactive caspase
• UniProt Gene Name: Casp12
• UniProt Entry Name: CASPC_RAT

NCBI Description

cysteine-aspartic acid protease (caspase); involved with the terminal stage of apoptosis [RGD]

Uniprot Description

CASP12: Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF. Detected in heart, kidney, liver, lung, pancreas, small intestine, spleen, stomach, thymus and testis. Belongs to the peptidase C14A family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.22.-; Protease

Cellular Component: intracellular membrane-bound organelle; endoplasmic reticulum; intracellular; cytosol; nucleus

Molecular Function: protein binding; protease binding; cysteine-type endopeptidase activity; endopeptidase activity; cysteine-type peptidase activity

Biological Process: response to drug; response to nicotine; response to inorganic substance; response to anoxia; positive regulation of apoptosis; apoptosis; response to iron(III) ion; unfolded protein response; positive regulation of caspase activity; response to organic nitrogen; proteolysis; regulation of apoptosis; protein autoprocessing; response to ethanol; regulation of inflammatory response; response to cold; aging