Rabbit anti-Human DLX4 Polyclonal Antibody | anti-DLX4 antibody

DLX4, ID (DLX4, BP1, DLX7, DLX8, DLX9, Homeobox protein DLX-4, Beta protein 1, Homeobox protein DLX-7, Homeobox protein DLX-8) (Biotin)

Cat. Num. AAA6292379

• Gene Names: DLX4; BP1; DLX7; DLX8; DLX9; OFC15
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DLX4; Polyclonal Antibody; ID (DLX4; BP1; DLX7; DLX8; DLX9; Homeobox protein DLX-4; Beta protein 1; Homeobox protein DLX-7; Homeobox protein DLX-8) (Biotin); Homeobox protein DLX-8; anti-DLX4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 240

• Applicable Applications for anti-DLX4 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: DLX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71-100 amino acids from the Central region of human DLX4.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DLX4 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the DLX4 antibody detected the DLX4 protein (arrow).)

Western Blot (WB)

(Western blot analysis of DLX4 (arrow) using rabbit polyclonal DLX4 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DLX4 gene.)

Related Product Information for anti-DLX4 antibody

Many vertebrate homeo box-containing genes have been identified on the basis of their sequence similarity with Drosophila developmental genes. Members of the Dlx gene family contain a homeobox that is related to that of Distal-less (Dll), a gene expressed in the head and limbs of the developing fruit fly. The Distal-less (Dlx) family of genes comprises at least 6 different members, DLX1-DLX6. The DLX proteins are postulated to play a role in forebrain and craniofacial development. Three transcript variants have been described for this gene, however, the full length nature of one variant has not been described. Studies of the two splice variants revealed that one encoded isoform functions as a repressor of the beta-globin gene while the other isoform lacks that function.

Product Categories/Family for anti-DLX4 antibody

Antibodies; Transcription Factors; Zinc; Ring; Homeobox

References

Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010): Schwartz, A.M., et al. Mod. Pathol. 22(1):1-6(2009). Man, Y.G., et al. Cancer Biomark 5(1):9-17(2009). Cavalli, L.R., et al. Cancer Genet. Cytogenet. 187(1):19-24(2008). Vieira, A.R., et al. Genet. Med. 10(9):668-674(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 20143962
• NCBI GeneID: 1748
• NCBI Accession #: NP_612138.1; NP_001925.2
• NCBI GenBank Nucleotide #: NP_612138.1
• UniProt Accession #: Q92988
• NCBI Official Full Name: homeobox protein DLX-4 isoform a
• NCBI Official Synonym Full Names: distal-less homeobox 4
• NCBI Official Symbol: DLX4
• NCBI Official Synonym Symbols: BP1; DLX7; DLX8; DLX9; OFC15
• NCBI Protein Information: homeobox protein DLX-4
• UniProt Protein Name: Homeobox protein DLX-4
• Protein Family: Homeobox protein
• UniProt Gene Name: DLX4
• UniProt Synonym Gene Names: BP1; DLX7; DLX8; DLX9
• UniProt Entry Name: DLX4_HUMAN

NCBI Description

Many vertebrate homeo box-containing genes have been identified on the basis of their sequence similarity with Drosophila developmental genes. Members of the Dlx gene family contain a homeobox that is related to that of Distal-less (Dll), a gene expressed in the head and limbs of the developing fruit fly. The Distal-less (Dlx) family of genes comprises at least 6 different members, DLX1-DLX6. The DLX proteins are postulated to play a role in forebrain and craniofacial development. Three transcript variants have been described for this gene, however, the full length nature of one variant has not been described. Studies of the two splice variants revealed that one encoded isoform functions as a repressor of the beta-globin gene while the other isoform lacks that function. [provided by RefSeq, Jul 2008]

Uniprot Description

DLX4: May play a role in determining the production of hemoglobin S. May act as a repressor. Belongs to the distal-less homeobox family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 17q21.33

Cellular Component: nucleus

Molecular Function: protein binding; sequence-specific DNA binding; transcription factor activity

Biological Process: regulation of transcription, DNA-dependent; multicellular organismal development; negative regulation of transcription from RNA polymerase II promoter