Western Blot (WB)
(Western Blot analysis of extracts from Jurkat cells treated with anti-CD3 antibody (1ug/ml for 10 minutes), using MBS628729 (upper) or control FAK antibody (lower).)
Rabbit anti-Human FAK Polyclonal Antibody
FAK, phosphorylated (Tyr925)
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Immunofluorescence
Purity
Affinity PurifiedPurified by Protein A and peptide affinity chromatography.
Synonyms
FAK; Polyclonal Antibody; phosphorylated (Tyr925); Anti -FAK; anti-FAK antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes endogenous levels of human FAK, phosphorylated (Tyr925) at ~125kD. Species Sequence Homology: mouse, rat and chicken.
Purity/Purification
Affinity Purified
Purified by Protein A and peptide affinity chromatography.
Purified by Protein A and peptide affinity chromatography.
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Applicable Applications for anti-FAK antibody
Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
Application Notes
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunofluorescence.
Dilution: Western Blot: 1:1000
Immunoprecipitation: 1:100
Immunofluorescence (IC): 1:50
Dilution: Western Blot: 1:1000
Immunoprecipitation: 1:100
Immunofluorescence (IC): 1:50
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Tyr925 of human FAK.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of extracts from Jurkat cells treated with anti-CD3 antibody (1ug/ml for 10 minutes), using MBS628729 (upper) or control FAK antibody (lower).)
Western Blot (WB)
(Western Blot analysis of extracts from Jurkat cells treated with anti-CD3 antibody (1ug/ml for 10 minutes), using MBS628729 (upper) or control FAK antibody (lower).)
Related Product Information for anti-FAK antibody
Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in integrin-mediated signal transduction. It plays an important role in the control of several biological processes, including cell spreading, migration and survival. Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397, which is a binding site for Src family kinases, PI3K and PLCgamma. The recruitment of Src family kinases results in the phosphorylation of tyrosine residues 407, 576 and 577 in the catalytic domain, and tyrosine residues 871 and 925 in the C-terminal region of FAK. Phosphorylation of Tyr925 creates a binding site for the Grb2/SH2 domain and triggers a Ras-dependent activation of the MAP kinase pathway.
Product Categories/Family for anti-FAK antibody
Similar Products
Product Notes
The FAK (Catalog #AAA628729) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FAK, phosphorylated (Tyr925) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FAK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF). Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunofluorescence. Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (IC): 1:50. Researchers should empirically determine the suitability of the FAK for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FAK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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