Western Blot (WB)
(Western Blot analysis of extracts from hydrogen peroxide treated or serum starved THP1 cells using MBS603108.)
Mouse anti-Human Btk Monoclonal Antibody
Btk, phosphorylated (Ser180) (Bruton's Tyrosine Kinase)
Reactivity
Human
Applications
ELISA, Western Blot
Purity
AscitesAscites
Synonyms
Btk; Monoclonal Antibody; phosphorylated (Ser180) (Bruton's Tyrosine Kinase); Anti -Btk; anti-Btk antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3i5
Specificity
Recognizes transfected levels of human Btk only when phosphorylated at Ser180 at ~80kD.
Purity/Purification
Ascites
Ascites
Ascites
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Applicable Applications for anti-Btk antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1:1000; For Western Blot, MyBioSource recommends using immunoprecipitated Btk.
Dilution: Western Blot: 1:1000; For Western Blot, MyBioSource recommends using immunoprecipitated Btk.
Immunogen
Synthetic phosphopeptide corresponding to residues around Ser180 of human Btk. Species Sequence Homology: mouse, 100%.
Species Sequence Homology
Mouse, 100%.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of extracts from hydrogen peroxide treated or serum starved THP1 cells using MBS603108.)
Western Blot (WB)
(Western Blot analysis of extracts from hydrogen peroxide treated or serum starved THP1 cells using MBS603108.)
Related Product Information for anti-Btk antibody
Bruton's tyrosine kinase (Btk) is a member of the Btk/Tec family of cytoplasmic tyrosine kinases. Like other Btk family members, it contains a pleckstrin homology (PH) domain, Src homology SH3 and SH2 domains. Btk plays an important role in B cell development. Activation of B cells by various ligands is accompanied by Btk membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane-located Btk is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation. The activation of Btk is negatively regulated by PKCbeta through phosphorylation of Btk at Ser180, which results in reduced membrane recruitment, transphosphorylation and subsequent activation. The PKC/Btk inhibitory signal is likely to be a key determinant of the B-cell receptor signaling threshold to maintain optimal Btk activity.
Product Categories/Family for anti-Btk antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The Btk (Catalog #AAA603108) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Btk, phosphorylated (Ser180) (Bruton's Tyrosine Kinase) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Btk can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: Western Blot: 1:1000; For Western Blot, MyBioSource recommends using immunoprecipitated Btk. Researchers should empirically determine the suitability of the Btk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Btk, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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