Western Blot (WB)
(Western blot analysis of extracts from TF-1 cells, starved for 18 hours, and treated with GM-CSF (25 ng/ml) for 10 minutes, using Phospho-Jak2 (Tyr1002/1003) (upper) or control Jak2 (lower).)
Rabbit anti-Human Jak2 Polyclonal Antibody | anti-jak2b antibody
Jak2, Phosphorylated (Tyr1007/1008) (Janus Kinase 1)
Gene Names
jak2b; jak2; JAK2bCA
Reactivity
Human
Applications
Western Blot
Purity
Affinity PurifiedPurified by protein A and peptide affinity chromatography.
Synonyms
Jak2; Polyclonal Antibody; Phosphorylated (Tyr1007/1008) (Janus Kinase 1); Anti -Jak2; anti-jak2b antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Detects endogenous levels of Jak2 only when phosphorylated at tyrosine 1007/1008. Species Crossreacactivity: Human, mouse, predicted to react with rat.
Purity/Purification
Affinity Purified
Purified by protein A and peptide affinity chromatography.
Purified by protein A and peptide affinity chromatography.
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.
Applicable Applications for anti-jak2b antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000
Dilution: Western Blot: 1:1000
Immunogen
Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr1007/1008 of human Jak2.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of extracts from TF-1 cells, starved for 18 hours, and treated with GM-CSF (25 ng/ml) for 10 minutes, using Phospho-Jak2 (Tyr1002/1003) (upper) or control Jak2 (lower).)
Western Blot (WB)
(Western blot analysis of extracts from TF-1 cells, starved for 18 hours, and treated with GM-CSF (25 ng/ml) for 10 minutes, using Phospho-Jak2 (Tyr1002/1003) (upper) or control Jak2 (lower).)
Related Product Information for anti-jak2b antibody
The Janus family tyrosine kinases, consisting of Jak1, Jak2, Jak3 and Tyk2, associate with a variety of cytokine receptors and are activated by ligand binding to these receptors (1). Upon ligand binding to cytokine receptors, Jaks phosphorylate themselves and their associated receptors, thereby providing multiple binding sites for signaling proteins containing SH2 or other phospho-tyrosine-binding domains, including Stats (2), Shc (3), insulin receptor substrates (4) and focal adhesion kinase (5). The tyrosine residues 1007/1008 of Jak2 in the putative activation loop are the homologous tyrosine residues 1054/1055 in Tyk2, which are important in the regulation of Tyk2 kinase activity (6).
Product Categories/Family for anti-jak2b antibody
NCBI and Uniprot Product Information
Research Articles on jak2b
Similar Products
Product Notes
The jak2b (Catalog #AAA620233) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Jak2, Phosphorylated (Tyr1007/1008) (Janus Kinase 1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Jak2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the jak2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Jak2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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