Western blot
(Western blot analysis of HUVECs untreated (lanes 1 & 3) or treated with alkaline phosphatase (lanes 2 & 4). Blots were probed with mouse monoclonal anti-FAK (lanes 1 & 2) and anti-FAK (Tyr-397) (lanes 3 & 4).)
Mouse FAK (Tyr-397) Monoclonal Antibody
FAK (Tyr-397), phospho-specific, Mouse Monoclonal IgG1
Reactivity
Human, Rat, Mouse, Rabbit
Applications
ELISA, Western Blot
Purity
Protein A Purified
Synonyms
FAK (Tyr-397); Monoclonal Antibody; phospho-specific; Mouse Monoclonal IgG1; Focal Adhesion Kinase 1; pp125FAK; anti-FAK (Tyr-397) antibody
Host
Mouse
Reactivity
Human, Rat, Mouse, Rabbit
Clonality
Monoclonal
Isotype
IgG1
Specificity
The antibody detects a 125 kDa* protein on SDS-PAGE immunoblots of untreated HUVEC cells. This phosphorylated band is greatly reduced after treatment with alkaline phosphatase.
Purity/Purification
Protein A Purified
Form/Format
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100ml phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-FAK (Tyr-397) antibody
ELISA, Western Blot
Application Notes
ELISA: 1:2000
Western Blot: 1:500
Western Blot: 1:500
Immunogen
Clone (M121) was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 397 of human FAK.
This peptide sequence has high homology to the conserved tyrosine site in rat and mouse FAK.
Buffer
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
Preparation and Storage
Store at –20°C.
Western blot
(Western blot analysis of HUVECs untreated (lanes 1 & 3) or treated with alkaline phosphatase (lanes 2 & 4). Blots were probed with mouse monoclonal anti-FAK (lanes 1 & 2) and anti-FAK (Tyr-397) (lanes 3 & 4).)
Western blot
(Western blot analysis of HUVECs untreated (lanes 1 & 3) or treated with alkaline phosphatase (lanes 2 & 4). Blots were probed with mouse monoclonal anti-FAK (lanes 1 & 2) and anti-FAK (Tyr-397) (lanes 3 & 4).)
Related Product Information for anti-FAK (Tyr-397) antibody
Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in signal transduction pathways important for cell spreading, migration and survival. Activation of FAK by integrin clustering leads to autophosphorylation at Tyr-397, which is a binding site for Src family kinases, PI3-Kinase, and PLC-gamma. The recruitment of Src family kinases results in the phosphorylation of tyrosine 407, 576, and 577 in the catalytic domain, and tyrosine 871 and 925 in the carboxy-terminal region of FAK. Thus, the phosphorylation of Tyr-397 is a critical step in the activation of FAK.
References
• Cobb, B.S. et al. (1994) Mol. Cell. Biol. 14:147.
• Parsons, J.T. et al. (2000) Oncogene 19:5606.
• Schaller, M.D. et al. (1994) Mol. Cell. Biol. 14:1680.
• Schlaepfer, D.D. et al. (1994) Nature 372:786-791.
• Parsons, J.T. et al. (2000) Oncogene 19:5606.
• Schaller, M.D. et al. (1994) Mol. Cell. Biol. 14:1680.
• Schlaepfer, D.D. et al. (1994) Nature 372:786-791.
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Product Notes
The FAK (Tyr-397) (Catalog #AAA470013) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The FAK (Tyr-397), phospho-specific, Mouse Monoclonal IgG1 reacts with Human, Rat, Mouse, Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's FAK (Tyr-397) can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. ELISA: 1:2000 Western Blot: 1:500. Researchers should empirically determine the suitability of the FAK (Tyr-397) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FAK (Tyr-397), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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