Sheep anti-Human, Mouse Semaphorin 3F Polyclonal Antibody | anti-SEMA3F antibody

Semaphorin 3F (Biotin)

Cat. Num. AAA626722

• Gene Names: SEMA3F; SEMA4; SEMAK; SEMA-IV
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Semaphorin 3F; Polyclonal Antibody; Semaphorin 3F (Biotin); Anti -Semaphorin 3F (Biotin); anti-SEMA3F antibody

• Host: Sheep
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Semaphorin 3F. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, 50ug/1ug BSA. Labeled with Biotin. Reconstitute with sterile TBS, pH 7.3, 20mM Trizma, 150mM sodium chloride, 0.1% BSA.

• Applicable Applications for anti-SEMA3F antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 0.1-0.2ug/ml
• Immunogen: Purified, NS0-derived, recombinant mouse Semaphorin 3F.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-SEMA3F antibody

The semaphorins make up a family of inhibitory axon guidance molecules that include secreted, transmembrane, and GPI anchored extracellular molecules. Via their interaction with Plexin/Neuropilin receptor complexes, the semaphorins are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. Semaphorin 3F belongs to the class 3 secreted group of molecules and shows ubiquitous expression in a range of neuronal and non-neuronal tissues including brain, mammary gland, liver, kidney, lung, spleen, muscle and heart. The role of the semaphorins in non-neuronal tissues is poorly characterised.

Product Categories/Family for anti-SEMA3F antibody

Antibodies; Abs to Plexin; Semaphorin

NCBI and Uniprot Product Information

• NCBI GI #: 188497703
• NCBI GeneID: 6405
• NCBI Accession #: NP_004177.3
• NCBI GenBank Nucleotide #: NM_004186.3
• UniProt Accession #: Q13275; Q13274; Q13372; Q15704; Q6GTR4; Q59G50
• Molecular Weight: 88,381 Da
• NCBI Official Full Name: semaphorin-3F
• NCBI Official Synonym Full Names: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F
• NCBI Official Symbol: SEMA3F
• NCBI Official Synonym Symbols: SEMA4; SEMAK; SEMA-IV
• NCBI Protein Information: semaphorin-3F; sema IV; sema III/F; semaphorin IV; semaphorin III/F; OTTHUMP00000210657; OTTHUMP00000210658; OTTHUMP00000210669; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, 3F
• UniProt Protein Name: Semaphorin-3F
• Protein Family: Semaphorin
• UniProt Gene Name: SEMA3F
• UniProt Entry Name: SEM3F_HUMAN

NCBI Description

The semaphorins are a family of proteins that are involved in signaling. All the family members have a secretion signal, a 500-amino acid sema domain, and 16 conserved cysteine residues (Kolodkin et al., 1993 [PubMed 8269517]). Sequence comparisons have grouped the secreted semaphorins into 3 general classes, all of which also have an immunoglobulin domain. The semaphorin III family, consisting of human semaphorin III (SEMA3A; MIM 603961), chicken collapsin, and mouse semaphorins A, D, and E, all have a basic domain at the C terminus. Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones (Luo et al., 1993 [PubMed 8402908]) through an interaction with a collapsin response mediator protein of relative molecular mass 62K (CRMP62) (Goshima et al., 1995 [PubMed 7637782]), a putative homolog of an axonal guidance associated UNC33 gene product (MIM 601168). SEMA3F is a secreted member of the semaphorin III family.[supplied by OMIM]

Uniprot Description

SEMA3F: May play a role in cell motility and cell adhesion. Belongs to the semaphorin family.

Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: extracellular space; membrane

Molecular Function: chemorepellent activity; receptor activity

Biological Process: axon extension involved in axon guidance; trigeminal nerve structural organization; facial nerve structural organization; axon guidance; branchiomotor neuron axon guidance; negative chemotaxis; nerve development; negative regulation of axon extension involved in axon guidance