Human Semaphorin 3F (SEMA3F) ELISA Kit | SEMA3F elisa kit

Human Semaphorin 3F (SEMA3F) ELISA Kit

Cat. Num. AAA2022622

• Gene Names: SEMA3F; SEMA4; SEMAK; SEMA-IV
• Reactivity: Human
• Synonyms: Semaphorin 3F (SEMA3F); Human Semaphorin 3F (SEMA3F) ELISA Kit; SEMA-IV; SEMA4; SEMAK; Sema Domain; Immunoglobulin Domain(Ig); Transmembrane Domain(TM)and Short Cytoplasmic Domain 3F; SEMA3F elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of SEMA3F. No significant cross-reactivity or interference between SEMA3F and analogues was observed.
• Sequence Length: 754

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15.6-1,000pg/mL
• Sensitivity: < 5.9pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SEMA3F were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SEMA3F were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for SEMA3F elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SEMA3F in human serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to SEMA3F. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to SEMA3F. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SEMA3F, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SEMA3F in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for SEMA3F elisa kit

CD & Adhesion molecule

NCBI and Uniprot Product Information

• NCBI GI #: 188497703
• NCBI GeneID: 6405
• NCBI Accession #: NP_004177.3
• NCBI GenBank Nucleotide #: NM_004186.3
• UniProt Accession #: Q13275; Q13274; Q13372; Q15704; Q6GTR4; C9JQ85
• Molecular Weight: 85,064 Da
• NCBI Official Full Name: semaphorin-3F
• NCBI Official Synonym Full Names: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F
• NCBI Official Symbol: SEMA3F
• NCBI Official Synonym Symbols: SEMA4; SEMAK; SEMA-IV
• NCBI Protein Information: semaphorin-3F
• UniProt Protein Name: Semaphorin-3F
• Protein Family: Semaphorin
• UniProt Gene Name: SEMA3F
• UniProt Synonym Gene Names: Sema IV
• UniProt Entry Name: SEM3F_HUMAN

NCBI Description

The semaphorins are a family of proteins that are involved in signaling. All the family members have a secretion signal, a 500-amino acid sema domain, and 16 conserved cysteine residues (Kolodkin et al., 1993 [PubMed 8269517]). Sequence comparisons have grouped the secreted semaphorins into 3 general classes, all of which also have an immunoglobulin domain. The semaphorin III family, consisting of human semaphorin III (SEMA3A; MIM 603961), chicken collapsin, and mouse semaphorins A, D, and E, all have a basic domain at the C terminus. Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones (Luo et al., 1993 [PubMed 8402908]) through an interaction with a collapsin response mediator protein of relative molecular mass 62K (CRMP62) (Goshima et al., 1995 [PubMed 7637782]), a putative homolog of an axonal guidance associated UNC33 gene product (MIM 601168). SEMA3F is a secreted member of the semaphorin III family.[supplied by OMIM, Mar 2008]

Uniprot Description

SEMA3F: May play a role in cell motility and cell adhesion. Belongs to the semaphorin family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: extracellular space; membrane

Molecular Function: chemorepellent activity; receptor activity

Biological Process: axon extension involved in axon guidance; axon guidance; facial nerve structural organization; trigeminal nerve structural organization; branchiomotor neuron axon guidance; negative chemotaxis; nerve development; negative regulation of axon extension involved in axon guidance