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Western Blot (WB) (Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Human fetal brain tissue and Human kidney tissue, Primary antibody: 123764(IDO2 Antibody) at dilution 1/450, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds)

Rabbit anti-Human IDO-2 Polyclonal Antibody | anti-IDO-2 antibody

IDO-2 Rabbit pAb

Gene Names
IDO2; INDOL1
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
IDO-2; Polyclonal Antibody; IDO-2 Rabbit pAb; indoleamine 2; 3-dioxygenase 2; anti-IDO-2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Affinity purified
Form/Format
pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Applicable Applications for anti-IDO-2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:2000
ELISA: 1:5000-1:10,000
IHC: 1:30-1:150
Immunogen
Full length fusion protein
Conjugation
Unconjugated
Modification
Unmodified
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Human fetal brain tissue and Human kidney tissue, Primary antibody: 123764(IDO2 Antibody) at dilution 1/450, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds)

Western Blot (WB) (Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Human fetal brain tissue and Human kidney tissue, Primary antibody: 123764(IDO2 Antibody) at dilution 1/450, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human brain tissue using 123764(IDO2 Antibody) at dilution 1/45. (Original magnification: ×200))

Immunohistochemistry (IHC) (The image is immunohistochemistry of paraffin-embedded Human brain tissue using 123764(IDO2 Antibody) at dilution 1/45. (Original magnification: ×200))
Related Product Information for anti-IDO-2 antibody
InDOL1 is also known as IDO2 (indoleamine 2, 3-dioxygenase 2) and is a 407 amino acid protein that is expressed in various tissues, including liver, small intestine, spleen, placenta, thymus, lung, brain, kidney, colon and dendritic cells. INDOL1 is selectively inhibited by D-1MT (1-methyl-d-tryptophan), which also inhibits IDO (indoleamine 2, 3-dioxygenase) and is significant because IDO expression causes suppression of T cell responses to tumors in dendritic cells. The inhibition of INDOL1 by D-1MT suggests a common function in immunomodulation. In the human INDOL1 gene, two single nucleotide polymorphisms have been detected which abolish the enzymatic function of INDOL1.
Product Categories/Family for anti-IDO-2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
19,610 Da
NCBI Official Full Name
Indoleamine 2,3-dioxygenase 2
NCBI Official Synonym Full Names
indoleamine 2,3-dioxygenase 2
NCBI Official Symbol
IDO2
NCBI Official Synonym Symbols
INDOL1
NCBI Protein Information
indoleamine 2,3-dioxygenase 2; IDO-2; indoleamine 2,3-dioxygenase-like 1 protein; indoleamine 2,3-dioxygenase-like protein 1; indoleamine-pyrrole 2,3 dioxygenase-like 1; indoleamine-pyrrole 2,3-dioxygenase-like protein 1
UniProt Protein Name
Indoleamine 2,3-dioxygenase 2
UniProt Gene Name
IDO2
UniProt Synonym Gene Names
INDOL1; IDO-2
UniProt Entry Name
I23O2_HUMAN

NCBI Description

Along with the enzymes encoded by the INDO (MIM 147435) and TDO2 (MIM 191070) genes, the enzyme encoded by the INDOL1 gene metabolizes tryptophan in the kynurenine pathway (Ball et al., 2007 [PubMed 17499941]).[supplied by OMIM, Feb 2011]

Uniprot Description

INDOL1: Catalyzes the first and rate-limiting step in the kynurenine pathway of tryptophan catabolism. Belongs to the indoleamine 2,3-dioxygenase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - tryptophan; Oxidoreductase; EC 1.13.11.-

Chromosomal Location of Human Ortholog: 8p11.21

Cellular Component: cytoplasm; cytosol

Molecular Function: metal ion binding; heme binding; indoleamine 2,3-dioxygenase activity; tryptophan 2,3-dioxygenase activity

Biological Process: tryptophan catabolic process to kynurenine; tryptophan catabolic process

Research Articles on IDO-2

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Product Notes

The IDO-2 ido2 (Catalog #AAA4754553) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IDO-2 Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IDO-2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 IHC: 1:30-1:150. Researchers should empirically determine the suitability of the IDO-2 ido2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IDO-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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