Semaphorin 3F (SEMA3F) Recombinant Protein | SEMA3F recombinant protein

Recombinant Semaphorin 3F (SEMA3F)

Cat. Num. AAA2011488

• Gene Names: SEMA3F; SEMA4; SEMAK; SEMA-IV
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation, Immunoprecipitation
• Purity: > 95%
• Synonyms: Semaphorin 3F (SEMA3F); Recombinant Semaphorin 3F (SEMA3F); SEMA3F recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; V RNPVIYAVFT SSGSVFRGSA VCVYSMADIR MVFNGPFAHK EGPNYQWMPF SGKMPYPRPG TCPGGTFTPS MKSTKDYPDE VINFMRSHPL MYQAVYPLQR RPLVVRTGAP YRLTTIAVDQ VDAADGRYEV LFL
• Sequence Length: 785

• Applicable Applications for SEMA3F recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), CoIP, Reporter Assays, Purification, Amine Reactive Labeling.
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Val350~Leu486
• Predicted Molecular Mass: 16.6kDa
• Accurate Molecular Mass: 16kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Predicted Molecular Mass: 16.6kDa
• Tags: N-terminal His-Tag
• Homology: Mouse 96%, rat 96%
• Tissue Specificity: Breast, kidney, brain, lung, liver.
• Subcellular Location: Secreted.
• Traits: Freeze-dried powder
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 188497703
• NCBI GeneID: 6405
• NCBI Accession #: NP_004177.3
• NCBI GenBank Nucleotide #: NM_004186.3
• UniProt Accession #: Q13275; Q13274; Q13372; Q15704; Q6GTR4; C9JQ85
• NCBI Official Full Name: semaphorin-3F
• NCBI Official Synonym Full Names: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F
• NCBI Official Symbol: SEMA3F
• NCBI Official Synonym Symbols: SEMA4; SEMAK; SEMA-IV
• NCBI Protein Information: semaphorin-3F; sema IV; sema III/F; semaphorin IV; semaphorin III/F; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, 3F
• UniProt Protein Name: Semaphorin-3F
• Protein Family: Semaphorin
• UniProt Gene Name: SEMA3F
• UniProt Synonym Gene Names: Sema IV
• UniProt Entry Name: SEM3F_HUMAN

NCBI Description

The semaphorins are a family of proteins that are involved in signaling. All the family members have a secretion signal, a 500-amino acid sema domain, and 16 conserved cysteine residues (Kolodkin et al., 1993 [PubMed 8269517]). Sequence comparisons have grouped the secreted semaphorins into 3 general classes, all of which also have an immunoglobulin domain. The semaphorin III family, consisting of human semaphorin III (SEMA3A; MIM 603961), chicken collapsin, and mouse semaphorins A, D, and E, all have a basic domain at the C terminus. Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones (Luo et al., 1993 [PubMed 8402908]) through an interaction with a collapsin response mediator protein of relative molecular mass 62K (CRMP62) (Goshima et al., 1995 [PubMed 7637782]), a putative homolog of an axonal guidance associated UNC33 gene product (MIM 601168). SEMA3F is a secreted member of the semaphorin III family.[supplied by OMIM, Mar 2008]

Uniprot Description

SEMA3F: May play a role in cell motility and cell adhesion. Belongs to the semaphorin family.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: extracellular space; membrane

Molecular Function: chemorepellent activity; receptor activity

Biological Process: axon extension involved in axon guidance; axon guidance; trigeminal nerve structural organization; facial nerve structural organization; branchiomotor neuron axon guidance; negative chemotaxis; nerve development; negative regulation of axon extension involved in axon guidance