Rabbit anti-Human, Mouse PTPN3 Polyclonal Antibody | anti-PTPN3 antibody

PTPN3 (Protein Tyrosine Phosphatase Non-receptor Type 3, Protein Tyrosine Phosphatase H1, PTP-H1, PTPH1)

Cat. Num. AAA624401

• Gene Names: PTPN3; PTPH1; PTP-H1; DKFZp686N0569
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: PTPN3; Polyclonal Antibody; PTPN3 (Protein Tyrosine Phosphatase Non-receptor Type 3; Protein Tyrosine Phosphatase H1; PTP-H1; PTPH1); Anti -PTPN3 (Protein Tyrosine Phosphatase Non-receptor Type 3; anti-PTPN3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PTPN3. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-PTPN3 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:10-1:50
• Immunogen: Synthetic peptide selected from the center region of human PTPN3 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-PTPH1 Center Pab is used in Western blot to detect PTPH1 in mouse brain tissue lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PTPH1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Related Product Information for anti-PTPN3 antibody

Phosphorylation of receptors by protein kinases is a process that can be reversed by a group of enzymes called protein phosphatases. Coordinated control of kinases and phosphatases provides the cell with the capacity to rapidly switch between phosphorylated and dephosphorylated protein states in dynamic response to environmental stimuli. Activation of critical enzymes by kinase phosphorylation alone is not enough to provide adequate regulation - it is the combination with phosphatase dephosphorylation that effectively creates on/off switches to control cellular events. Errors in control, either through kinases or their counterpart phosphatases, can lead to unchecked cell growth attributable to human cancers and developmental disorders. Potential mechanisms to control dephosphorylation include changes in the expression of protein phosphatases, their subcellular localization, phosphorylation of phosphatase catalytic and regulatory subunits and regulation by endogenous phosphatase inhibitors. Most protein phosphatases are not stringently specific for their substrates. Consequently, changes in phosphatase activity may have a broad impact on dephosphorylation and turnover of phosphoproteins that are substrates for different kinases. This may be an important point of control to connect cellular circuitry of interrelated signaling pathways, and to synchronize physiological responses.

Product Categories/Family for anti-PTPN3 antibody

Antibodies; Abs to Enzymes; Phosphatase

NCBI and Uniprot Product Information

• NCBI GI #: 219520091
• NCBI GeneID: 5774
• UniProt Accession #: P26045; A0AUW9; Q45VJ3; Q68E08; Q8N4S3
• Molecular Weight: 103,990 Da
• NCBI Official Full Name: PTPN3 protein
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, non-receptor type 3
• NCBI Official Symbol: PTPN3
• NCBI Official Synonym Symbols: PTPH1; PTP-H1; DKFZp686N0569
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type 3; OTTHUMP00000021885; protein-tyrosine phosphatase H1; cytoskeletal-associated protein tyrosine phosphatase
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type 3
• Protein Family: Tyrosine-protein phosphatase non-receptor
• UniProt Gene Name: PTPN3
• UniProt Synonym Gene Names: PTPH1
• UniProt Entry Name: PTN3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This protein contains a C-terminal PTP domain and an N-terminal domain homologous to the band 4.1 superfamily of cytoskeletal-associated proteins. P97, a cell cycle regulator involved in a variety of membrane related functions, has been shown to be a substrate of this PTP. This PTP was also found to interact with, and be regulated by adaptor protein 14-3-3 beta. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

PTPN3: May act at junctions between the membrane and the cytoskeleton. Possesses tyrosine phosphatase activity. Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.

Protein type: Motility/polarity/chemotaxis; EC 3.1.3.48; Protein phosphatase, tyrosine (non-receptor)

Chromosomal Location of Human Ortholog: 9q31

Cellular Component: internal side of plasma membrane; cytoskeleton; cytoplasm

Molecular Function: protein binding; sodium channel regulator activity; phosphotyrosine binding; cytoskeletal protein binding; protein tyrosine phosphatase activity; ATPase binding

Biological Process: negative regulation of mitotic cell cycle; negative regulation of membrane protein ectodomain proteolysis; protein amino acid dephosphorylation