Rabbit MANF / ARMET Polyclonal Antibody | anti-MANF antibody

Rabbit Polyclonal to Human MANF / ARMET

Cat. Num. AAA242565

• Gene Names: MANF; ARP; ARMET
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: MANF / ARMET; Polyclonal Antibody; Rabbit Polyclonal to Human MANF / ARMET; Anti-MANF / ARMET Antibody (C-Terminus) IHC-plus; MANF; ARP; ARMET; Arginine-rich protein; Protein ARMET; Human MANF; anti-MANF antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 185

• Applicable Applications for anti-MANF antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB (1 - 2 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: 12 amino acid peptide from near the carboxy terminus of human MANF.
• Immunogen: MANF / ARMET antibody was raised against 12 amino acid peptide from near the carboxy terminus of human MANF.
• Antigen Modification: C-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-MANF / ARMET antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of MANF in Human Brain cells with MANF antibody at 20 ug/ml.)

Western Blot (WB)

(Western blot of MANF in mouse brain tissue lysate with MANF antibody at (A) 1 and (B) 2 ug/ml. Below: Immunohistochemistry of MANF in human brain tissue with MANF antibody at 2.5 ug/ml.)

Related Product Information for anti-MANF antibody

The ARP gene encodes a highly conserved arginine-rich protein of unknown function. The presence of a specific mutation changing codon 50 from ATG to AGG or deletion of codon 50 of the ARP gene in different tumor types has been reported. In particular, mutation is associated with pancreatic cancer.

NCBI and Uniprot Product Information

• NCBI GI #: 54873600
• NCBI GeneID: 7873
• NCBI Accession #: NP_006001.2
• NCBI GenBank Nucleotide #: NM_006010.3
• UniProt Accession #: P55145; Q14CX4; Q86U67; Q96IS4
• Molecular Weight: 20,700 Da
• NCBI Official Full Name: mesencephalic astrocyte-derived neurotrophic factor
• NCBI Official Synonym Full Names: mesencephalic astrocyte-derived neurotrophic factor
• NCBI Official Symbol: MANF
• NCBI Official Synonym Symbols: ARP; ARMET
• NCBI Protein Information: mesencephalic astrocyte-derived neurotrophic factor; arginine-rich, mutated in early stage tumors
• UniProt Protein Name: Mesencephalic astrocyte-derived neurotrophic factor
• Protein Family: Mannan endo-1,4-beta-mannosidase
• UniProt Gene Name: MANF
• UniProt Synonym Gene Names: ARMET; ARP
• UniProt Entry Name: MANF_HUMAN

NCBI Description

The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and results in cell proliferation. Activity of this protein is important in promoting the survival of dopaminergic neurons. The presence of polymorphisms in the N-terminal arginine-rich region, including a specific mutation that changes an ATG start codon to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in normal tissues and are thus no longer thought to be tumor-related. [provided by RefSeq, Apr 2014]

Uniprot Description

ARMET: Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. Belongs to the ARMET family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: extracellular space; perinuclear region of cytoplasm; endoplasmic reticulum; nucleus

Molecular Function: growth factor activity

Biological Process: vasoconstriction of artery involved in ischemic response to lowering of systemic arterial blood pressure; response to unfolded protein

Disease: Pancreatic Cancer