Rabbit anti-Human, Mouse PTPN3 Polyclonal Antibody | anti-PTPN3 antibody

PTPN3 (Protein Tyrosine Phosphatase Non-receptor Type 3, Protein Tyrosine Phosphatase H1, PTP-H1, PTPH1) (Biotin)

Cat. Num. AAA6495721

• Gene Names: PTPN3; PTPH1; PTP-H1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: PTPN3; Polyclonal Antibody; PTPN3 (Protein Tyrosine Phosphatase Non-receptor Type 3; Protein Tyrosine Phosphatase H1; PTP-H1; PTPH1) (Biotin); anti-PTPN3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PTPN3. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-PTPN3 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human PTPN3 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-PTPH1 Center Pab is used in Western blot to detect PTPH1 in mouse brain tissue lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PTPH1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-PTPN3 antibody

Antibodies; Enzymes; Phosphatase

References

1. Ikuta, S., et al., J. Gastroenterol. 29(6):727-732 (1994). 2. Arimura, Y., et al., Tumour Biol. 13(3):180-186 (1992). 3. Yang, Q., et al., Proc. Natl. Acad. Sci. USA 88(14):5949-5953 (1991).

NCBI and Uniprot Product Information

• NCBI GI #: 223941879
• NCBI GeneID: 5774
• NCBI Accession #: NP_001138840.1
• NCBI GenBank Nucleotide #: NM_001145368.1
• UniProt Accession #: P26045; A0AUW9; E7EN99; E9PGU7
• Molecular Weight: 83,723 Da
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type 3 isoform 2
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, non-receptor type 3
• NCBI Official Symbol: PTPN3
• NCBI Official Synonym Symbols: PTPH1; PTP-H1
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type 3
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type 3
• Protein Family: Tyrosine-protein phosphatase non-receptor
• UniProt Gene Name: PTPN3
• UniProt Synonym Gene Names: PTPH1; PTP-H1

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This protein contains a C-terminal PTP domain and an N-terminal domain homologous to the band 4.1 superfamily of cytoskeletal-associated proteins. P97, a cell cycle regulator involved in a variety of membrane related functions, has been shown to be a substrate of this PTP. This PTP was also found to interact with, and be regulated by adaptor protein 14-3-3 beta. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]

Uniprot Description

May act at junctions between the membrane and the cytoskeleton. Possesses tyrosine phosphatase activity.