Rabbit anti-Human, mouse PTP alpha Polyclonal Antibody | anti-PTPRA antibody

PTP alpha Antibody (N-term)

Cat. Num. AAA9214843

• Gene Names: PTPRA; LRP; HLPR; PTPA; HEPTP; HPTPA; RPTPA; PTPRL2; HPTPalpha; R-PTP-alpha
• Reactivity: Human, mouse
• Applications: Western Blot, ELISA
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: PTP alpha; Polyclonal Antibody; PTP alpha Antibody (N-term); Receptor-type tyrosine-protein phosphatase alpha; Protein-tyrosine phosphatase alpha; R-PTP-alpha; PTPRA; PTPA; PTPRL2; anti-PTPRA antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This PTP alpha antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-120 amino acids from the N-terminal region of human PTP alpha.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 89-120
• Sequence Length: 793

• Applicable Applications for anti-PTPRA antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(The anti-PTPalpha N-term Pab is used in Western blot to detect PTPalpha in mouse brain tissue lysate.)

Related Product Information for anti-PTPRA antibody

Phosphorylation of receptors by protein kinases is a process that can be reversed by a group of enzymes called protein phosphatases. Coordinated control of kinases and phosphatases provides the cell with the capacity to rapidly switch between phosphorylated and dephosphorylated protein states in dynamic response to environmental stimuli. Activation of critical enzymes by kinase phosphorylation alone is not enough to provide adequate regulation ?it is the combination with phosphatase dephosphorylation that effectively creates on/off switches to control cellular events. Errors in control, either through kinases or their counterpart phosphatases, can lead to unchecked cell growth attributable to human cancers and developmental disorders. Potential mechanisms to control dephosphorylation include changes in the expression of protein phosphatases, their subcellular localization, phosphorylation of phosphatase catalytic and regulatory subunits and regulation by endogenous phosphatase inhibitors. Most protein phosphatases are not stringently specific for their substrates. Consequently, changes in phosphatase activity may have a broad impact on dephosphorylation and turnover of phosphoproteins that are substrates for different kinases. This may be an important point of control to connect cellular circuitry of interrelated signaling pathways, and to synchronize physiological responses.

Product Categories/Family for anti-PTPRA antibody

Signal Transduction

References

Deloukas, P., et al., Nature 414(6866):865-871 (2001).
Kaplan, R., et al., Proc. Natl. Acad. Sci. U.S.A. 87(18):7000-7004 (1990).
Krueger, N.X., et al., EMBO J. 9(10):3241-3252 (1990).
Sap, J., et al., Proc. Natl. Acad. Sci. U.S.A. 87(16):6112-6116 (1990).
Jirik, F.R., et al., FEBS Lett. 273 (1-2), 239-242 (1990).

NCBI and Uniprot Product Information

• NCBI GI #: 4506303
• NCBI GeneID: 5786
• NCBI Accession #: NP_002827.1; NP_543030.1; NP_543031.1
• NCBI GenBank Nucleotide #: NM_002836.3
• UniProt Accession #: P18433; Q14513; Q7Z2I2; Q96TD9; A8K2G8; D3DVX5
• Molecular Weight: 90600
• NCBI Official Full Name: receptor-type tyrosine-protein phosphatase alpha isoform 1
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, receptor type, A
• NCBI Official Symbol: PTPRA
• NCBI Official Synonym Symbols: LRP; HLPR; PTPA; HEPTP; HPTPA; RPTPA; PTPRL2; HPTPalpha; R-PTP-alpha
• NCBI Protein Information: receptor-type tyrosine-protein phosphatase alpha
• UniProt Protein Name: Receptor-type tyrosine-protein phosphatase alpha
• Protein Family: Receptor-type tyrosine-protein phosphatase
• UniProt Gene Name: PTPRA
• UniProt Synonym Gene Names: PTPA; PTPRL2; Protein-tyrosine phosphatase alpha; R-PTP-alpha
• UniProt Entry Name: PTPRA_HUMAN

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. This PTP has been shown to dephosphorylate and activate Src family tyrosine kinases, and is implicated in the regulation of integrin signaling, cell adhesion and proliferation. Three alternatively spliced variants of this gene, which encode two distinct isoforms, have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

PTPRA: a receptor protein tyrosine phosphatase (PTP). Contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. Dephosphorylates and activates Src family tyrosine kinases, and is implicated in the regulation of integrin signaling, cell adhesion and proliferation. Four alternatively spliced isoforms have been reported.

Protein type: EC 3.1.3.48; Motility/polarity/chemotaxis; Membrane protein, integral; Receptor protein phosphatase, tyrosine

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: integral to plasma membrane; plasma membrane; receptor complex

Molecular Function: protein binding; transmembrane receptor protein tyrosine phosphatase activity; protein tyrosine phosphatase activity

Biological Process: axon guidance; insulin receptor signaling pathway; protein amino acid phosphorylation