Western Blot (WB)
(Western blot analysis of extracts from NIH/3T3 and C6 cells starved for 16 hours and treated with TPA (0.2uM) for 30 minutes.)
Rabbit MARCKS Polyclonal Antibody | anti-LOC396473 antibody
MARCKS, phosphorylated (Ser152/156) (Myristolated Alanine Rich C Kinase Substrate, Myristoylated Alanine Rich Protein Kinase C Substrate, MACS, MARCS, 80K-L Protein, 81kD Protein Light Chain, MGC52672, Phosphomyristin, PKCSL, PRKCSL, Protein Kinase C Subs
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
MARCKS; Polyclonal Antibody; phosphorylated (Ser152/156) (Myristolated Alanine Rich C Kinase Substrate; Myristoylated Alanine Rich Protein Kinase C Substrate; MACS; MARCS; 80K-L Protein; 81kD Protein Light Chain; MGC52672; Phosphomyristin; PKCSL; PRKCSL; Protein Kinase C Subs; Anti -MARCKS; anti-LOC396473 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Detects endogenous levels of Ser152/156 phosphorylated MARCKS. Species Crossreactivity: Human and mouse.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Applicable Applications for anti-LOC396473 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Dilution: Western Blotting: 1:1000
Immunogen
Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser152/156 of rat MARCKS.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of extracts from NIH/3T3 and C6 cells starved for 16 hours and treated with TPA (0.2uM) for 30 minutes.)
Western Blot (WB)
(Western blot analysis of extracts from NIH/3T3 and C6 cells starved for 16 hours and treated with TPA (0.2uM) for 30 minutes.)
Related Product Information for anti-LOC396473 antibody
Myristoylated alanine-rich protein kinase C substrate (MARCKS) is a major PKC substrate that is distributed in various cell types. MARCKS has been implicated in cell motility, cell adhesion, phagocytosis, membrane traffic and mitogenesis (1). PKC phosphorylates serines 152, 156 and 163 of MARCKS (2), which regulates MARCKS's calcium/calmodulin binding activity and filamentous (F)-actin cross-linking activity (3,4). In addition, phosphorylation by PKC results in translocation of MARCKS from membrane to cytoplasm (5).
Product Categories/Family for anti-LOC396473 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
27,728 Da
NCBI Official Full Name
myristoylated alanine-rich C-kinase substrate
NCBI Official Symbol
LOC396473
NCBI Protein Information
myristoylated alanine-rich C-kinase substrate; MARCKS
UniProt Protein Name
Myristoylated alanine-rich C-kinase substrate
Protein Family
UniProt Gene Name
MARCKS
UniProt Entry Name
MARCS_CHICK
Uniprot Description
MARCKS: MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein
Protein type: Actin-binding; Motility/polarity/chemotaxis
Cellular Component: cytoskeleton; membrane; cytoplasm
Molecular Function: calmodulin binding; actin binding
Research Articles on LOC396473
Similar Products
Product Notes
The LOC396473 marcks (Catalog #AAA622252) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MARCKS, phosphorylated (Ser152/156) (Myristolated Alanine Rich C Kinase Substrate, Myristoylated Alanine Rich Protein Kinase C Substrate, MACS, MARCS, 80K-L Protein, 81kD Protein Light Chain, MGC52672, Phosphomyristin, PKCSL, PRKCSL, Protein Kinase C Subs reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MARCKS can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Dilution: Western Blotting: 1:1000. Researchers should empirically determine the suitability of the LOC396473 marcks for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MARCKS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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