Human KLK5 ELISA Kit | KLK5 elisa kit

Human KLK5 PicoKine ELISA Kit

Cat. Num. AAA175871

• Gene Names: KLK5; SCTE; KLKL2; KLK-L2
• Reactivity: Human
• Synonyms: KLK5; Human KLK5 PicoKine ELISA Kit; Kallikrein-5; kallikrein-related peptidase 5; Kallikrein 5; Kallikrein-like protein 2; KLK L2; SCTE; Stratum corneum tryptic enzyme; KLK5 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human KLK5
• Sequence Length: 293

• Samples: Cell culture supernates, serum, plasma (heparin, EDTA), human milk and saliva
• Assay Type: Sandwich
• Detection Range: 78pg/ml-5,000pg/ml
• Sensitivity: < 10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human KLK5 PicoKine ELISA Kit standard curve )

Related Product Information for KLK5 elisa kit

Principle of the assay: human KLK5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for KLK5 has been precoated onto 96-well plates. Standards(NSO, V23-S293) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KLK5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human KLK5 amount of sample captured in plate.
Background: Kallikrein gene 5 (KLK5, also known as KLK-L2), located on chromosome 19q13.4, is one of the newly identified members of the kallikrein gene family, which is a subgroup of the serine protease enzyme family. In normal human tissues, KLK5 is highly expressed in skin, mammary gland and testis. KLK5 has been suggested to regulate cell shedding (desquamation) in conjunction with KLK7 and KLK14, given its ability to degrade proteins which form the extracellular component of cell junctions in the stratum corneum. It is proposed that KLK5 regulates this process since it is able to self-activate in addition to activating KLK7 and KLK14.

NCBI and Uniprot Product Information

• NCBI GI #: 296434569
• NCBI GeneID: 25818
• UniProt Accession #: Q9Y337; Q53ZR3; Q9HBG8
• Molecular Weight: 32,019 Da
• NCBI Official Full Name: Kallikrein-5
• NCBI Official Synonym Full Names: kallikrein-related peptidase 5
• NCBI Official Symbol: KLK5
• NCBI Official Synonym Symbols: SCTE; KLKL2; KLK-L2
• NCBI Protein Information: kallikrein-5; kallikrein 5; kallikrein-like protein 2; stratum corneum tryptic enzyme
• UniProt Protein Name: Kallikrein-5
• Protein Family: Kallikrein
• UniProt Gene Name: KLK5
• UniProt Synonym Gene Names: SCTE; KLK-L2
• UniProt Entry Name: KLK5_HUMAN

NCBI Description

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its expression is up-regulated by estrogens and progestins. The encoded protein is secreted and may be involved in desquamation in the epidermis. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

KLK5: May be involved in desquamation. Belongs to the peptidase S1 family. Kallikrein subfamily.

Protein type: EC 3.4.21.-; Secreted, signal peptide; Secreted; Protease

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: extracellular space

Molecular Function: peptidase activity; protein binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: epidermis development; positive regulation of G-protein coupled receptor protein signaling pathway; proteolysis