Mouse KLK1 ELISA Kit | Klk1 elisa kit

Mouse KLK1 PicoKine ELISA Kit

Cat. Num. AAA178770

• Gene Names: Klk1; Kal; Klk6; KAL-B; mGk-6; Klk1b6; 0610007D04Rik
• Reactivity: Mouse
• Synonyms: KLK1; Mouse KLK1 PicoKine ELISA Kit; Kallikrein 1; Kallikrein1; Kallikrein-1; klk 1; Klk 6; Klk-1; Klk1; P06870; Pancreatic kallikrein; Klk1 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse KLK1
• Sequence Length: 213

• Samples: Cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA)
• Assay Type: Quantitative Sandwich
• Detection Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <12pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Mouse KLK1 PicoKine ELISA Kit standard curve )

Related Product Information for Klk1 elisa kit

Principle of the Assay: The Mouse KLK1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for KLK1 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: I25-D261) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KLK1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin- Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse KLK1 amount of sample captured in plate.

Background: Kallikrein-1, also known as tissue kallikrein, is a protein that in humans is encoded by the KLK1 gene. This serine protease generates Lys-bradykinin by specific proteolysis of kininogen-1. KLK1 is a member of the peptidase S1 family. Its gene is mapped to 19q13.3. In all, it has got 262-amino acids which contain a putative signal peptide, followed by a short activating peptide and the protease domain. The protein is mainly found in kidney, pancreas, and salivary gland, showing a unique pattern of tissue-specific expression relative to other members of the family. KLK1 is implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers.

NCBI and Uniprot Product Information

• NCBI GI #: 998614215
• NCBI GeneID: 16612
• NCBI Accession #: NP_001307260.1
• NCBI GenBank Nucleotide #: NM_001320331.1
• UniProt Accession #: P15947; Q61855
• Molecular Weight: 28,775 Da
• NCBI Official Full Name: kallikrein-1 isoform 2
• NCBI Official Synonym Full Names: kallikrein 1
• NCBI Official Symbol: Klk1
• NCBI Official Synonym Symbols: Kal; Klk6; KAL-B; mGk-6; Klk1b6; 0610007D04Rik
• NCBI Protein Information: kallikrein-1
• UniProt Protein Name: Kallikrein-1
• Protein Family: Blarinasin
• UniProt Gene Name: Klk1
• UniProt Synonym Gene Names: Klk-6; Klk6; mGK-6

NCBI Description

This gene encodes a member of the kallikrein subfamily of serine proteases that are involved in diverse physiological functions such as skin desquamation, tooth enamel formation, seminal liquefaction, synaptic neural plasticity and brain function. The encoded preproprotein undergoes proteolytic cleavage of the activation peptide to generate the functional enzyme. Mice lacking the encoded protein are unable to generate significant levels of kinins in most tissues, develop cardiovascular abnormalities and exhibit hypercalciuria of renal origin. This gene is located in a cluster of several related kallikrein genes on chromosome 7. Alternative splicing results in multiple transcript variants encoding different isoforms, some of which may undergo similar processing. [provided by RefSeq, Feb 2016]

Uniprot Description

KLK1: Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. Belongs to the peptidase S1 family. Kallikrein subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.21.35; Protease

Chromosomal Location of Human Ortholog: 7 B3|7 28.74 cM

Cellular Component: extracellular space; nucleus; protein complex

Molecular Function: endopeptidase activity; hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; serine-type endopeptidase activity