Western Blot (WB)
(WB: NIH 3T3 cell membrane extract probed with MBS620279 at 1:1000 dilution)
Rabbit Gi alpha 1,2 Polyclonal Antibody
Gi alpha 1,2
Reactivity
Bovine, Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation, Immunocytochemistry
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
Gi alpha 1; 2; Polyclonal Antibody; Anti -Gi alpha 1; anti-Gi alpha 1; 2 antibody
Host
Rabbit
Reactivity
Bovine, Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes rat Gia-1 and Gia-2, Mr 40kD. Does not crossreact with Gia-3 or Goa. Species Crossreactivity: mouse
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added.
Applicable Applications for anti-Gi alpha 1,2 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
Application Notes
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry.
Dilution: Western Blot: 1:500-1:1000 dilution detected the Gia-1 and Gia-2 protein in 40ug rat brain microsomal preparation and in 10ug of solubilized plasma membranes from mouse 3T3/A31 fibroblasts. The rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with G2033-90 (1:1000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunocytochemistry: 1:500 dilution gave positive immunostaining of mouse 3T3/A31 fibroblasts fixed for 3 minutes with ice-cold 100% acetone.
Optimal dilution determined by the researcher.
Dilution: Western Blot: 1:500-1:1000 dilution detected the Gia-1 and Gia-2 protein in 40ug rat brain microsomal preparation and in 10ug of solubilized plasma membranes from mouse 3T3/A31 fibroblasts. The rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with G2033-90 (1:1000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunocytochemistry: 1:500 dilution gave positive immunostaining of mouse 3T3/A31 fibroblasts fixed for 3 minutes with ice-cold 100% acetone.
Optimal dilution determined by the researcher.
Immunogen
Synthetic peptide (KNNLKDCGLF) corresponding to the C-terminal region shared by rat Gia-1 and Gia-2, coupled to KLH.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(WB: NIH 3T3 cell membrane extract probed with MBS620279 at 1:1000 dilution)
Western Blot (WB)
(WB: NIH 3T3 cell membrane extract probed with MBS620279 at 1:1000 dilution)
Product Categories/Family for anti-Gi alpha 1,2 antibody
NCBI and Uniprot Product Information
NCBI GI #
Similar Products
Product Notes
The Gi alpha 1,2 (Catalog #AAA620279) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Gi alpha 1,2 reacts with Bovine, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Gi alpha 1,2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC). Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Dilution: Western Blot: 1:500-1:1000 dilution detected the Gia-1 and Gia-2 protein in 40ug rat brain microsomal preparation and in 10ug of solubilized plasma membranes from mouse 3T3/A31 fibroblasts. The rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with G2033-90 (1:1000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunocytochemistry: 1:500 dilution gave positive immunostaining of mouse 3T3/A31 fibroblasts fixed for 3 minutes with ice-cold 100% acetone. Optimal dilution determined by the researcher. Researchers should empirically determine the suitability of the Gi alpha 1,2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Gi alpha 1,2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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