Western Blot (WB)
(Western Blot: Sample: Human K562 cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse ASNS Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Mouse Asparagine Synthetase Polyclonal Antibody | anti-ASNS antibody
Polyclonal Antibody to Asparagine Synthetase (ASNS)
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Human K562 cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse ASNS Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Human K562 cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse ASNS Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Mouse Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Mouse Tissue))
NCBI and Uniprot Product Information
Uniprot Description
ASNS: asparagine synthetase, an ubiquitous enzyme in mammalian organs, is involved in the first step of the synthesis of L-asparagine from L-glutamine and L-aspartate. Highly regulated in response to cell stress. Induced by dietary protein deficiency, imbalanced amino acid intake via the amino acid response, or ER stress via the EIF2AK3-eIF2-ATF4 branch of the unfolded protein response (UPR). Expression induced by nutrient deprivation or hypoxia in solid tumors. Essential for brain development but apparently not for that of other organs. Deficiency impairs brain development resulting in microcephaly and progressive cerebral atrophy, as well as deficiencies in learning and memory. The mechanism through which ASNS induces neuronal damage may be via damage induced by excessive excitability induced by elevated levels of aspartate/glutamate, via asparagine deficiency, or both. Three isoforms of the human protein are produced by alternative splicing.
Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Cell cycle regulation; Cell development/differentiation; EC 6.3.5.4; Energy Metabolism - nitrogen; Ligase
Chromosomal Location of Human Ortholog: 6|6 A1
Cellular Component: cytosol
Molecular Function: asparagine synthase (glutamine-hydrolyzing) activity; ATP binding; cofactor binding; ligase activity; nucleotide binding; protein homodimerization activity
Biological Process: amino acid biosynthetic process; asparagine biosynthetic process; cellular response to glucose starvation; glutamine metabolic process; negative regulation of apoptosis; positive regulation of mitotic cell cycle
Research Articles on ASNS
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Product Notes
The ASNS asns (Catalog #AAA2028225) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Asparagine Synthetase (ASNS) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Asparagine Synthetase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the ASNS asns for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Asparagine Synthetase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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