Human Signaling Lymphocytic Activation Molecule Family, Member 7 (SLAMF7) ELISA Kit | SLAMF7 elisa kit

Human Signaling Lymphocytic Activation Molecule Family, Member 7 (SLAMF7) ELISA Kit

Cat. Num. AAA453571

• Gene Names: SLAMF7; 19A; CS1; CD319; CRACC
• Reactivity: Human
• Synonyms: Signaling Lymphocytic Activation Molecule Family; Member 7 (SLAMF7); Human Signaling Lymphocytic Activation Molecule Family; Member 7 (SLAMF7) ELISA Kit; CD319; CRACC; 19A; CS1; CD2-like receptor-activating cytotoxic cells; Membrane protein FOAP-12; SLAMF7 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of SLAMF7. No significant cross-reactivity or interference between SLAMF7 and analogues was observed.
• Sequence Length: 165

• Samples: Human tissue homogenates, cell lysates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: <0.053ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SLAMF7 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SLAMF7 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SLAMF7 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of SLAMF7 in human tissue homogenates, cell lysates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SLAMF7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SLAMF7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SLAMF7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SLAMF7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 543583691
• NCBI GeneID: 57823
• NCBI Accession #: NP_001269517.1
• NCBI GenBank Nucleotide #: NM_001282588.1
• UniProt Accession #: Q9NQ25; Q8N6Y8; Q8ND32; Q9NY08; Q9NY23; A8K3U1; B4DPU4; B4DPY3; B4DWA3
• Molecular Weight: 21,316 Da
• NCBI Official Full Name: SLAM family member 7 isoform b
• NCBI Official Synonym Full Names: SLAM family member 7
• NCBI Official Symbol: SLAMF7
• NCBI Official Synonym Symbols: 19A; CS1; CD319; CRACC
• NCBI Protein Information: SLAM family member 7
• UniProt Protein Name: SLAM family member 7
• Protein Family: SLAM family
• UniProt Gene Name: SLAMF7
• UniProt Synonym Gene Names: CS1; CRACC

Uniprot Description

Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Isoform 1 mediates NK cell activation through a SH2D1A-independent extracellular signal-regulated ERK-mediated pathway (PubMed:11698418). Positively regulates NK cell functions by a mechanism dependent on phosphorylated SH2D1B. Downstream signaling implicates PLCG1, PLCG2 and PI3K (PubMed:16339536). In addition to heterotypic NK cells-target cells interactions also homotypic interactions between NK cells may contribute to activation. However, in the absence of SH2D1B, inhibits NK cell function. Acts also inhibitory in T-cells (). May play a role in lymphocyte adhesion (PubMed:11802771). In LPS-activated monocytes negatively regulates production of proinflammatory cytokines (PubMed:23695528).