Rabbit anti-Rat Aquaporin 8 Polyclonal Antibody | anti-AQP8 antibody

Aquaporin 8 (AQP8)

Cat. Num. AAA619753

• Gene Names: AQP8; AQP-8
• Reactivity: Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Aquaporin 8; Polyclonal Antibody; Aquaporin 8 (AQP8); Anti -Aquaporin 8 (AQP8); anti-AQP8 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat Aquaporin 8.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, pH 7.4, 0.1% BSA. Reconstitute with 100ul of PBS.

• Applicable Applications for anti-AQP8 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunofluorescence.; Dilution: Western Blot: 1-10ug/ml; ELISA: 0.5-1ug/ml; Immunohistochemistry and Immunofluorescence: 2-10ug/ml in a paraformaldehyde fixed sections of tissue.; Optimal dilution to be determined by researcher
• Immunogen: A 16 AA synthetic peptide within the C-terminal, cytoplasmic domain of rat Aquaporin-8 (Accession #P56405). Species sequence homology: 93% mouse, 81% human.
• Note: We recommend the use of 0.5-1% milk in all primary/secondary antibody-enzyme conjugate incubations in order to suppress non-specific bands
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-AQP8 antibody

Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. Aquaporin-0 or MIP26 (major intrinsic protein 26kD), and Aquaporin-1 (purified from red cells) also called CHIP-28 (channel forming integral protein, 28kD; 268 AA; gene locus 7p14) has been the foundation of the growing family of aquaporins. The lens specific Aquaporin-0 represents up to 80% of total lens membrane protein.

Product Categories/Family for anti-AQP8 antibody

Antibodies; Abs to Aquaporin

NCBI and Uniprot Product Information

• NCBI GI #: 6606076
• NCBI GeneID: 343
• UniProt Accession #: O94778; Q8IUU3; Q9UIA4; Q53GF6
• Molecular Weight: 27,381 Da
• NCBI Official Full Name: aquaporin 8
• NCBI Official Synonym Full Names: aquaporin 8
• NCBI Official Symbol: AQP8
• NCBI Official Synonym Symbols: AQP-8
• NCBI Protein Information: aquaporin-8; OTTHUMP00000122504
• UniProt Protein Name: Aquaporin-8
• Protein Family: Aquaporin
• UniProt Gene Name: AQP8
• UniProt Entry Name: AQP8_HUMAN

NCBI Description

Aquaporin 8 (AQP8) is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 8 mRNA is found in pancreas and colon but not other tissues. [provided by RefSeq]

Uniprot Description

AQP8: Forms a water-specific channel; mercury-sensitive. Not permeable to glycerol or urea. Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein type: Membrane protein, integral; Channel, misc.; Transporter; Transporter, aquaporin family; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 16p12

Cellular Component: intracellular canaliculus; intracellular membrane-bound organelle; integral to plasma membrane; apical part of cell; plasma membrane

Molecular Function: water channel activity; glycerol channel activity

Biological Process: glycerol transport; response to reactive oxygen species; cellular water homeostasis; transport; canalicular bile acid transport; water transport; transmembrane transport