Rabbit anti-Mouse, Human Hspa1b Polyclonal Antibody | anti-Hspa1b antibody

Hspa1b Antibody

Cat. Num. AAA968752

• Gene Names: Hspa1b; Hsp70; hsp68; Hsp70-1; Hsp70.1
• Reactivity: Mouse, Human
• Applications: ELISA, Western Blot
• Purity: >95%, Protein G Purified
• Synonyms: Hspa1b; Polyclonal Antibody; Hspa1b Antibody; Heat shock 70 kDa protein 1B; Hcp70.1; Hsp70-1; Hsp70a1; Hspa1; Heat shock 70 kDa protein 1; HSP70.1; anti-Hspa1b antibody

• Host: Rabbit
• Reactivity: Mouse, Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G Purified
• Sequence Length: 642

• Applicable Applications for anti-Hspa1b antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Recommended dilution: WB:1:500-2000
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugate: Non-conjugated
• Immunogen: Recombinant mouse Heat shock 70 kDa protein 1B protein
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: Hspa1b antibody at 5ug/mlLane 1: A431 whole cell lysateLane 2: A549 whole cell lysateLane 3: Jurkat whole cell lysateLane 4: MCF7 whole cell lysateLane 5: K562 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 70 kDaObserved band size: 70 kDa)

Related Product Information for anti-Hspa1b antibody

In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. Essential for STUB1-mediated ubiquitination and degradation of FOXP3 in regulatory T-cells (Treg) during inflammation.

NCBI and Uniprot Product Information

• NCBI GI #: 124339826
• NCBI GeneID: 15511
• NCBI Accession #: NP_034608.2
• NCBI GenBank Nucleotide #: NM_010478.2
• UniProt Accession #: P17879; Q61689; Q925V6
• Molecular Weight: 70,176 Da
• NCBI Official Full Name: heat shock 70 kDa protein 1B
• NCBI Official Synonym Full Names: heat shock protein 1B
• NCBI Official Symbol: Hspa1b
• NCBI Official Synonym Symbols: Hsp70; hsp68; Hsp70-1; Hsp70.1
• NCBI Protein Information: heat shock 70 kDa protein 1B
• UniProt Protein Name: Heat shock 70 kDa protein 1B
• Protein Family: Heat shock 70 kDa protein
• UniProt Gene Name: Hspa1b
• UniProt Synonym Gene Names: Hcp70.1; Hsp70-1; Hsp70a1; Hspa1; HSP70.1
• UniProt Entry Name: HS71B_MOUSE

Uniprot Description

HSP70: a critical chaperone protein that has a high affinity for unfolded polypeptide chains. It binds extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In cooperation with other chaperones, hsp70 stabilizes preexistent proteins against aggregation and mediates the folding of newly translated polypeptides in the cytosol as well as within organelles. Mitochondrial HSP70 is crucial to the import process: mutant forms of HSP70 fail to import precursor proteins. Is anti-apoptotic in sympathetic neurones and mediates this effect primarily by suppressing c-Jun transcriptional signalling. Interacts with tau protein and mediates proper folding of tau. Can promote the degradation of tau protein. Triptolide, a potential therapeutic agent for progression/metastasis of pancreatic cancer, causes pancreatic cancer cell death by induction of apoptosis, an effect mediated by the inhibition of HSP70.

Protein type: Motility/polarity/chemotaxis; Heat shock protein; Chaperone

Cellular Component: apical plasma membrane; basolateral plasma membrane; centriole; cytoplasm; cytosol; focal adhesion; inclusion body; mitochondrion; nucleus; perinuclear region of cytoplasm; protein complex; ubiquitin ligase complex

Molecular Function: ATP binding; ATPase activity; ATPase activity, coupled; double-stranded RNA binding; enzyme binding; G-protein-coupled receptor binding; heat shock protein binding; histone deacetylase binding; NF-kappaB binding; protease binding; receptor binding; ubiquitin protein ligase binding; unfolded protein binding

Biological Process: DNA repair; response to heat; telomere maintenance