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Immunohistochemistry (IHC) (Peptide-+ Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MED21 antibody.)

anti-Human, Mouse MED21 Antibody | anti-MED21 antibody

MED21 Antibody

Gene Names
MED21; SRB7; SURB7; hSrb7
Reactivity
Human, Mouse
Applications
Immunohistochemistry, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
MED21; MED21 Antibody; Mediator of RNA polymerase II transcription subunit 21; Mediator complex subunit 21; RNA polymerase II holoenzyme component SRB7; RNAPII complex component SRB7; hSrb7; SRB7; SURB7; anti-MED21 antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse
Specificity
MED21 antibody detects endogenous levels of total MED21 protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
144
Applicable Applications for anti-MED21 antibody
Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
IHC: 1:50-1:100
ELISA: 1:5000
Immunogen
The antiserum was produced against synthesized peptide derived from internal of human MED21.
Preparation and Storage
Store at-20 degree C for 1 year.

Immunohistochemistry (IHC)

(Peptide-+ Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MED21 antibody.)

Immunohistochemistry (IHC) (Peptide-+ Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MED21 antibody.)
References
Chao D.M., Nature 380:82-85(1996). Maldonado E., Nature 381:86-89(1996). Oelgeschlaeger T., Mol. Cell 1:925-931(1998).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
15,564 Da
NCBI Official Full Name
mediator of RNA polymerase II transcription subunit 21 isoform 1
NCBI Official Synonym Full Names
mediator complex subunit 21
NCBI Official Symbol
MED21
NCBI Official Synonym Symbols
SRB7; SURB7; hSrb7
NCBI Protein Information
mediator of RNA polymerase II transcription subunit 21
UniProt Protein Name
Mediator of RNA polymerase II transcription subunit 21
UniProt Gene Name
MED21
UniProt Synonym Gene Names
SRB7; SURB7; RNAPII complex component SRB7; hSrb7

NCBI Description

This gene encodes a member of the mediator complex subunit 21 family. The encoded protein interacts with the human RNA polymerase II holoenzyme and is involved in transcriptional regulation of RNA polymerase II transcribed genes. A pseudogene of this gene is located on chromosome 8. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]

Uniprot Description

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Research Articles on MED21

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Product Notes

The MED21 med21 (Catalog #AAA851747) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The MED21 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MED21 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA). IHC: 1:50-1:100 ELISA: 1:5000. Researchers should empirically determine the suitability of the MED21 med21 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MED21, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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