Rabbit anti-Human NHERF1 Polyclonal Antibody | anti-SLC9A3R1 antibody

NHERF1 (Na+/H+ Exchanger Regulatory Factor, Ezrin-Radixin-Moesin Binding Protein 50)

Cat. Num. AAA615647

• Gene Names: SLC9A3R1; EBP50; NHERF; NHERF1; NHERF-1; NPHLOP2
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: NHERF1; Polyclonal Antibody; NHERF1 (Na+/H+ Exchanger Regulatory Factor; Ezrin-Radixin-Moesin Binding Protein 50); Anti -NHERF1 (Na+/H+ Exchanger Regulatory Factor; anti-SLC9A3R1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NHERF1.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.1% BSA.

• Applicable Applications for anti-SLC9A3R1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 0.5-1ug/ml. Coat ELISA plates at 1ug/ml. with N2025-02A Control peptide.; Western Blot: 1-10ug/ml using ECL technique.; Optimal dilution determined by the researcher.
• Immunogen: Synthetic peptide corresponding to 14aa from the C-terminal, cytoplasmic domain of Human NHERF1 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-SLC9A3R1 antibody

Na+/ H+ exchanger regulatory factor (NHERF1) is a PDZ domain containing adaptor protein known to bind to various receptors, channels, cytoskeletal elements and cytoplasmic signaling proteins. A 358aa mainly expressed in kidney, liver and pancrease by NHERF gene (chrm 17). And is represented in another isoform, NHERF2. NHERF1 protein contains 2 tandem PDZ domains of approximately 90aa and a C-terminal sequence that binds several members of the ERM (ezrin-radixin-moesin) family of membrane cytoskeletal adapters. The protein plays an important role in regulation of NHE3, turnover of G-protein coupled receptors, platelet derived growth factor receptor and ion transporters such as CFTR, Na/Pi cotransporter, NaHCO3 cotransporter and Trp channels. NHERF1 overexpression in cancers and mutations in NHERF1 targets, Merlin, the product of tumor suppressor gene, also expressed in 2 isoforms, Merlin is involved in pathogenesis of benign tumors of the human nervous system, Merlin is named for its striking similarity with ERM family which thought to link cytoskeletal components with proteins in the cell membrane, It has a binding partner called Syntenin, which is an adapter protein that couples transmembrane proteoglycans to cytoskeletal components.

Product Categories/Family for anti-SLC9A3R1 antibody

Antibodies; Abs to Ion Transport

NCBI and Uniprot Product Information

• NCBI GI #: 4759140
• NCBI GeneID: 9368
• NCBI Accession #: NP_004243.1
• NCBI GenBank Nucleotide #: NM_004252.4
• UniProt Accession #: O14745; O43552; Q86WQ5
• Molecular Weight: 38,868 Da
• NCBI Official Full Name: Na(+)/H(+) exchange regulatory cofactor NHE-RF1
• NCBI Official Synonym Full Names: solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1
• NCBI Official Symbol: SLC9A3R1
• NCBI Official Synonym Symbols: EBP50; NHERF; NHERF1; NHERF-1; NPHLOP2
• NCBI Protein Information: Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Na+/H+ exchange regulatory co-factor; regulatory cofactor of Na(+)/H(+) exchanger; ezrin-radixin-moesin binding phosphoprotein-50; ezrin-radixin-moesin-binding phosphoprotein 50; solute carrier family 9 isoform A3 regulatory factor 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1
• UniProt Protein Name: Na(+)/H(+) exchange regulatory cofactor NHE-RF1
• Protein Family: Na(+)/H(+) exchange regulatory cofactor
• UniProt Gene Name: SLC9A3R1
• UniProt Synonym Gene Names: NHERF; NHERF1; NHERF-1; EBP50
• UniProt Entry Name: NHRF1_HUMAN

NCBI Description

This gene encodes a sodium/hydrogen exchanger regulatory cofactor. The protein interacts with and regulates various proteins including the cystic fibrosis transmembrane conductance regulator and G-protein coupled receptors such as the beta2-adrenergic receptor and the parathyroid hormone 1 receptor. The protein also interacts with proteins that function as linkers between integral membrane and cytoskeletal proteins. The protein localizes to actin-rich structures including membrane ruffles, microvilli, and filopodia. Mutations in this gene result in hypophosphatemic nephrolithiasis/osteoporosis type 2, and loss of heterozygosity of this gene is implicated in breast cancer.[provided by RefSeq, Sep 2009]

Uniprot Description

NHERF: a scaffold protein that connects plasma membrane proteins with members of the Ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized beta-2-adrenergic receptor Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: centrosome; microvillus; intracellular membrane-bound organelle; brush border membrane; endomembrane system; lipid raft; actin cytoskeleton; ruffle; membrane; microvillus membrane; cytoplasm; apical plasma membrane; plasma membrane; nucleus; vesicle; filopodium

Molecular Function: protein binding; chloride channel regulator activity; protein self-association; beta-2 adrenergic receptor binding; beta-catenin binding; protein complex binding; dopamine receptor binding; protein complex scaffold; receptor binding; phosphatase binding; PDZ domain binding

Biological Process: bile acid secretion; Wnt receptor signaling pathway; negative regulation of protein kinase B signaling cascade; renal sodium ion transport; microvillus biogenesis; negative regulation of cell proliferation; cellular phosphate ion homeostasis; regulation of sodium:hydrogen antiporter activity; protein complex assembly; dopamine receptor, phospholipase C activating pathway; regulation of protein kinase activity; actin cytoskeleton organization and biogenesis; negative regulation of sodium:hydrogen antiporter activity; negative regulation of cell migration; dopamine receptor, adenylate cyclase activating pathway; negative regulation of phosphoinositide 3-kinase cascade

Disease: Nephrolithiasis/osteoporosis, Hypophosphatemic, 2