Western Blot (WB)
(Western blot analysis of EBP50 on MCF-7 cells lysates using anti-EBP50 antibody at 1/1, 000 dilution.)
Rabbit anti-Human, Rat EBP50 Monoclonal Antibody | anti-EBP50 antibody
EBP50 Antibody
Gene Names
SLC9A3R1; EBP50; NHERF; NHERF1; NHERF-1; NPHLOP2
Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry
Purity
ProA affinity purified
Synonyms
EBP50; Monoclonal Antibody; EBP50 Antibody; EBP 50 antibody; EBP50 antibody; Ezrin radixin moesin binding phosphoprotein 50 antibody; Ezrin-radixin-moesin-binding phosphoprotein 50 antibody; Na (+)/H (+) exchange regulatory cofactor NHE RF antibody; Na (+)/H (+) exchange regulatory cofactor NHE-RF1 antibody; Na+/H+ exchange regulatory co factor antibody; NHERF 1 antibody; NHERF antibody; NHERF-1 antibody; NHERF1 antibody; NHRF1_HUMAN antibody; NPHLOP2 antibody; Regulatory cofactor of Na (+)/H (+) exchanger antibody; Slc9a3r1 antibody; Sodium hydrogen exchanger regulatory factor 1 antibody; Sodium-hydrogen exchanger regulatory factor 1 antibody; Sodium/hydrogen exchanger regulatory factor 1 antibody; Solute carrier family 9 (sodium/hydrogen exchanger) member 3 regulator 1 antibody; Solute carrier family 9 (sodium/hydrogen exchanger); isoform 3 regulatory factor 1 antibody; Solute carrier family 9 isoform 3 regulatory factor 1 antibody; Solute carrier family 9 isoform A3 regulatory factor 1 antibody; Solute carrier family 9 member 3 regulator 1 antibody; anti-EBP50 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Monoclonal
Clone Number
JJ0946
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-EBP50 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:1000-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western blot analysis of EBP50 on MCF-7 cells lysates using anti-EBP50 antibody at 1/1, 000 dilution.)
Western Blot (WB)
(Western blot analysis of EBP50 on MCF-7 cells lysates using anti-EBP50 antibody at 1/1, 000 dilution.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-EBP50 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-EBP50 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-EBP50 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-EBP50 antibody. Counter stained with hematoxylin.)
Related Product Information for anti-EBP50 antibody
The Na+/H+ exchange protein (NHE3) functions in transepithelial Na+ absorption and is primarily expressed in the intestinal and renal brush border membrane. NHE3 regulatory factor 1 (NHERF-1) interacts with NHE3 through two PDZ (for PSD-95, Discs-large and ZO-1 homology) domains, which are protein-protein interaction modules that associate with specific carboxy-terminal motifs on target proteins. Also known as EBP50, NHERF-1 facilitates cAMP inhibition of NHE3 to decrease Na+ adsorption. NHERF-1 functions as a scaffold for an essential multiprotein complex of Ezrin and NHE3 for cAMP-mediated phosphorylation and consequent inhibition of NHE3. The amino-terminal PDZ domain regulates the dimerization of NHERF-1 in vivo. G protein-coupled receptor kinase 6A phosphorylates NHERF-1 at Ser 289 via a PDZ domain-mediated interaction. NHERF-2, also known as E3KARP, is a ubiquitously expressed protein which also functions in NHE2 regulation.
Product Categories/Family for anti-EBP50 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
358
NCBI Official Full Name
Na(+)/H(+) exchange regulatory cofactor NHE-RF1
NCBI Official Synonym Full Names
solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1
NCBI Official Symbol
SLC9A3R1
NCBI Official Synonym Symbols
EBP50; NHERF; NHERF1; NHERF-1; NPHLOP2
NCBI Protein Information
Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Na+/H+ exchange regulatory co-factor; regulatory cofactor of Na(+)/H(+) exchanger; ezrin-radixin-moesin binding phosphoprotein-50; ezrin-radixin-moesin-bindi
UniProt Protein Name
Na(+)/H(+) exchange regulatory cofactor NHE-RF1
UniProt Gene Name
SLC9A3R1
UniProt Synonym Gene Names
NHERF; NHERF1; NHERF-1; EBP50
UniProt Entry Name
NHRF1_HUMAN
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Product Notes
The EBP50 slc9a3r1 (Catalog #AAA9436058) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EBP50 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's EBP50 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:1000-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the EBP50 slc9a3r1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EBP50, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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