Rabbit Nherf1 Polyclonal Antibody | anti-SLC9A3R1 antibody

Nherf1 (Na(+)/H(+) Exchange Regulatory Cofactor NHE-RF1, NHERF-1, Ezrin-radixin-moesin-binding Phosphoprotein 50, EBP50, Regulatory Cofactor of Na(+)/H(+) Exchanger, Sodium-hydrogen Exchanger Regulatory Factor 1, Solute Carrier Family 9 Isoform A3 Regulat

Cat. Num. AAA648062

• Gene Names: SLC9A3R1; EBP50; NHERF; NHERF1; NHERF-1; NPHLOP2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: Nherf1; Polyclonal Antibody; Nherf1 (Na(+)/H(+) Exchange Regulatory Cofactor NHE-RF1; NHERF-1; Ezrin-radixin-moesin-binding Phosphoprotein 50; EBP50; Regulatory Cofactor of Na(+)/H(+) Exchanger; Sodium-hydrogen Exchanger Regulatory Factor 1; Solute Carrier Family 9 Isoform A3 Regulat; Anti -Nherf1 (Na(+)/H(+) Exchange Regulatory Cofactor NHE-RF1; anti-SLC9A3R1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat NHERF1/EBP50. Species Crossreactivity: human and mouse.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.5% BSA, 5mM EDTA, 0.01% thimerosal, 30% glycerol.

• Applicable Applications for anti-SLC9A3R1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1-4ug/ml
• Immunogen: Synthetic peptide corresponding to aa338 from rat NHERF1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of NHERF1 using rat kidney tissue lysate.)

Related Product Information for anti-SLC9A3R1 antibody

NHE3 regulatory factor 1 (NHERF-1) is a multifunctional adaptor protein that interacts with NHE3 via its PDZ domains. The amino-terminal PDZ domain regulates the dimerization of NHERF-1 in vivo. G protein-coupled receptor kinase 6A phosphorylates NHERF-1 at Ser 289 via a PDZ domain-mediated interaction.

Product Categories/Family for anti-SLC9A3R1 antibody

Antibodies; Abs to Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4759140
• NCBI GeneID: 9368
• NCBI Accession #: NP_004243.1
• NCBI GenBank Nucleotide #: NM_004252.4
• UniProt Accession #: O14745; O43552; Q86WQ5
• Molecular Weight: 38,868 Da
• NCBI Official Full Name: Na(+)/H(+) exchange regulatory cofactor NHE-RF1
• NCBI Official Synonym Full Names: solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1
• NCBI Official Symbol: SLC9A3R1
• NCBI Official Synonym Symbols: EBP50; NHERF; NHERF1; NHERF-1; NPHLOP2
• NCBI Protein Information: Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Na+/H+ exchange regulatory co-factor; regulatory cofactor of Na(+)/H(+) exchanger; ezrin-radixin-moesin binding phosphoprotein-50; ezrin-radixin-moesin-binding phosphoprotein 50; solute carrier family 9 isoform A3 regulatory factor 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1
• UniProt Protein Name: Na(+)/H(+) exchange regulatory cofactor NHE-RF1
• Protein Family: Na(+)/H(+) exchange regulatory cofactor
• UniProt Gene Name: SLC9A3R1
• UniProt Synonym Gene Names: NHERF; NHERF1; NHERF-1; EBP50
• UniProt Entry Name: NHRF1_HUMAN

NCBI Description

This gene encodes a sodium/hydrogen exchanger regulatory cofactor. The protein interacts with and regulates various proteins including the cystic fibrosis transmembrane conductance regulator and G-protein coupled receptors such as the beta2-adrenergic receptor and the parathyroid hormone 1 receptor. The protein also interacts with proteins that function as linkers between integral membrane and cytoskeletal proteins. The protein localizes to actin-rich structures including membrane ruffles, microvilli, and filopodia. Mutations in this gene result in hypophosphatemic nephrolithiasis/osteoporosis type 2, and loss of heterozygosity of this gene is implicated in breast cancer.[provided by RefSeq, Sep 2009]

Uniprot Description

Function: Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli

By similarity. Involved in the regulation of phosphate reabsorption in the renal proximal tubules. Involved in sperm capacitation. May participate in the regulation of the chloride and bicarbonate homeostasis in spermatozoa. Ref.2 Ref.6 Ref.7 Ref.40

Subunit structure: Homodimer, and heterodimer with SLC9A3R2. Binds the N-termini of EZR, RDX and MSN. Binds the C-termini of PDGFRA, PDGFRB, ADRB2, NOS2 and CFTR. Binds ARHGAP17, EPI64, GNB2L1, OPRK1, GNAQ, CTNNB1 and PLCB3. Binds PDZK1

By similarity. Interacts with CLCN3. Binds the C-terminus of PAG1. In resting T-cells, part of a PAG1-SLC9A3R1-MSN complex which is disrupted upon TCR activation. Forms a complex with CFTR and SLC4A7. Forms a complex with SLC4A7 and ATP6V1B1. Interacts with TRPC4 (via the PDZ-binding domain). Directly interacts with HTR4

By similarity. Interacts (via the PDZ 1 domain) with PODXL (via the C-terminal PDZ-binding motif DTHL); interaction is not detected in glomerular epithelium cells. Interacts (via the PDZ 1 domain) with PODXL (via the C-terminal PDZ-binding motif DTHL); the interaction take place early in the secretory pathway and is necessary for its apical membrane sorting

By similarity. Interacts with SLC26A3

By similarity. Interacts with MCC. Interacts with SLC34A1. Interacts (via the PDZ domains) with SLC26A6 isoform 4 and isoform 5. Ref.1 Ref.2 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.17 Ref.18 Ref.19 Ref.20 Ref.28 Ref.33 Ref.35

Subcellular location: Cytoplasm

By similarity. Apical cell membrane

By similarity. Endomembrane system; Peripheral membrane protein. Cell projection › filopodium. Cell projection › ruffle. Cell projection › microvillus. Note: Translocates from the cytoplasm to the apical cell membrane in a PODXL-dependent manner. Colocalizes with CFTR at the midpiece of sperm tail

By similarity. Colocalizes with actin in microvilli-rich apical regions of the syncytiotrophoblast. Found in microvilli, ruffling membrane and filopodia of HeLa cells. Present in lipid rafts of T-cells. Ref.1 Ref.2 Ref.8

Tissue specificity: Detected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast. Ref.1 Ref.6

Induction: By estrogen. Ref.16

Post-translational modification: Phosphorylated on serine residues. Ref.1

Involvement in disease: Nephrolithiasis/osteoporosis, hypophosphatemic, 2 (NPHLOP2) [MIM:612287]: A disease characterized by decreased renal phosphate absorption, renal phosphate wasting, hypophosphatemia, hyperphosphaturia, hypercalciuria, nephrolithiasis and osteoporosis.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.33 Ref.40

Sequence similarities: Contains 2 PDZ (DHR) domains.

Sequence caution: The sequence AAH49220.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.